基于高通量测序技术解析鲣鱼(Katsuwonus pelamis)鱼油和鱼白调控肠道菌群结构作用

REGULATION EFFECT OF TUNA(KATSUWONUS PELAMIS)FISH OIL AND FISH SPERM ON GUT MICROBIOTA STRUCTURE

本研究利用高通量测序技术分析小鼠喂食鲣鱼(Katsuwonus pelamis)鱼油和鱼白酶解液后肠道微生物的多样性、群落组成以及结构的变化,探讨高蛋白、高脂肪饮食对人类健康的影响。研究结果显示:喂食鱼油组小鼠的肠道微生物丰富且多样。在门水平上,对照组和鱼油组的优势菌门为拟杆菌门(Bacteroidetes);鱼白组的优势菌门为厚壁菌门(Firmicutes)。在科水平上,对照组和鱼油组中的优势菌科为Muribaculaceae;鱼白组中的优势菌科为毛螺菌科(Lachnospiraceae)。在属水平上,对照组和鱼油组中的优势菌属为Muribaculum;鱼白组中的优势菌为Lachnoclostridium;对照组、鱼油组和鱼白组小鼠的肠道微生物群落中瘤胃球菌属(Ruminococcus)和拟杆菌属(Bacteroides)等丰度较高。在种水平上,对照组和鱼油组中的优势菌为Muribaculum intestinale;鱼白组中的优势菌为解糖梭菌(Clostridium saccharolyticum)。研究结果证明鲣鱼鱼油和鱼白可以调节菌群结构和组成,增加肠道有益菌,减少病原菌的丰度等。

High-throughput sequencing technology was used to analyze the diversity,community composition,and structure of gut microbes in mice fed with tuna fish oil and fish sperm enzymatic hydrolysis solution.Based on the changes of the gut microbes,the impact of high-protein and high-fat diets on human health was discussed.The results show that the gut microbes of the mice fed with the fish oil group were rich and diverse.At the phylum level,the predominant phylum of the control group and fish oil group was Bacteroidetes;the predominant phylum of the fish sperm group was Firmicutes.At the family level,the dominant family in the control group and fish oil group was Muribaculaceae;the dominant family in the fish sperm group was Lachnospiraceae.At the genus level,the predominant genus in the control group and fish oil group was Muribaculum;the predominant genus in the fish sperm group was Lachnoclostridium;the intestinal microbial community of mice in the control group,fish oil group,and fish sperm group also included Ruminococcus and Bacteroides.At the species level,the dominant bacteria in the control group and fish oil group was Muribaculum intestinale;the dominant bacteria in the fish sperm group was Clostridium saccharolyticum.It was determined that tuna fish oil and fish sperm can regulate the structure and composition of the gut microbes,increase the gut beneficial bacteria,and reduce the abundance of pathogenic bacteria,etc.