基于网络药理学的黄芩-黄连治疗溃疡性结肠炎作用机制探讨

Mechanism of Scutellaria baicalensis Georgi-Coptis chinensis Franch in the treatment of ulcerative colitis based on network pharmacology

目的探讨黄芩-黄连治疗溃疡性结肠炎的作用机制。方法运用中药系统药理学数据库和分析平台(TCMSP)数据库获取黄芩-黄连药对有效成分及预测药物作用靶点;运用在线人类孟德尔遗传数据库(OMIM)数据库及人类基因数据库(GeneCards)获取溃疡性结肠炎疾病基因;得出交集靶标基因后运用STRING探讨其相关性,分析潜在作用机制。结果共收集到黄芩-黄连药对活性成分41种,活性成分预测靶标基因179个,溃疡性结肠炎的靶标基因411个,药物-疾病交集靶标基因41个,基因本体(GO)功能富集分析表明主要涉及信号传导、细胞增殖等生物过程,京都基因和基因组百科全书(KEGG)富集分析表明黄芩-黄连药对治疗溃疡性结肠炎主要通过癌症通路、TNF通路、IL-17通路及Toll样受体信号通路等发挥作用。结论黄芩-黄连治疗溃疡性结肠炎是通过多活性成分-多靶点-多通路的复杂过程,或可降低溃疡性结肠炎发展成为结直肠癌的风险。

Objective To explore the mechanism of Scutellaria baicalensis Georgi-Coptis chinensis Franch in the treatment of ulcerative colitis. Methods The effective components and predicted drug targets of Scutellaria baicalensis Georgi-Coptis chinensis Franch were obtained by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database;the ulcerative colitis disease genes were obtained by online human Mendelian genetic database(OMIM)and human gene database(GeneCards);the intersection target genes were obtained and the correlation and potential mechanism was discussed by STRING. Result A total of 41 active components,179 predicted target genes of active components,411 target genes of ulcerative colitis and 41 target genes of drug disease intersection were collected. Gene Ontology(GO)enrichment analysis showed that they were mainly involved in signal transduction,cell proliferation and other biological processes. Kyoto Encyclopedia of Genesand Genomes(KEGG)enrichment analysis showed that the treatment of ulcerative colitis by Scutellaria baicalensis Georgi-Coptis chinensis Franch was mainly through cancer pathway,TNF IL-17 pathway and toll like receptor signaling pathway to play a role. Conclusion The treatment of ulcerative colitis by Scutellaria baicalensis Georgi-Coptis chinensis Franch a complex process of multiple active components,multiple targets and multiple pathways,or can reduce the risk of ulcerative colitis developing into colorectal cancer.