应用碘元素自动检测仪测定尿中碘含量的方法评价实验

Evaluation of the method for determination of iodine content in urine with automatic iodine element analyzer

目的评价碘元素自动检测仪测定尿中碘含量方法的应用价值。方法应用DAT-50S碘元素自动检测仪测定尿中碘含量,对该方法进行方法学适用性实验,包括标准曲线以及检出限、精密度、标准物质测定、加标回收、方法对比实验等。结果在碘含量0~800μg/L范围内,标准曲线的相关系数为-0.9997,检出限为6.2μg/L。精密度:标准曲线范围内测定低、中、高3个碘含量尿样的相对标准偏差(RSD)分别为1.6%、1.1%、0.7%(n=8)。准确度:2个尿碘标准物质的测定值均在参考值范围内;标准曲线范围内低、中、高3个碘含量尿样的平均回收率为99.6%,范围为97.6%~101.2%。方法对比实验:与砷铈催化分光光度法(WS/T 107.1-2016)对比测定20份尿样,结果差异无统计学意义(t=0.984,P>0.05)。结论应用碘元素自动检测仪测定尿中碘含量的精密度、准确度均较高,操作简单,适宜在疾病预防控制机构推广应用。

Objective To study the method of measuring iodine content in urine with automatic iodine element analyzer.Methods The iodine content in urine was measured by automatic iodine element analyzer DAT-50S,and the applicability of the method was tested,including the standard curve and detection limit,precision test,standard substance determination test,standard addition recovery test,and method comparison test.Results Within the range of iodine content 0-800μg/L,the correlation coefficient of the standard curve was-0.9997,and the detection limit was 6.2μg/L.Precision:the relative standard deviation(RSD)values of three urine samples with low,medium and high iodine contents within the range of the standard curve were 1.6%,1.1%and 0.7%,respectively(n=8).Accuracy:the measured values of two urine iodine standard substances measured by the automatic iodine element analyzer were all within the reference value range;the average recovery rate of three urine samples with low,medium and high iodine contents within the range of the standard curve was 99.6%,and the recovery rate ranged from 97.6%to 101.2%.Method comparison test:there was no statistically significant difference between the results of 20 urine samples determined by arsenic-cerium catalytic spectrophotometry(WS/T 107.1-2016)and the automatic iodine element analyzer(t=0.984,P>0.05).Conclusion The precision and accuracy of urinary iodine determination using the automatic iodine element analyzer are high,the operation is simple,and it is suitable for popularization and application in grassroots disease control institutions.