光谱型电化学发光定量分析进展

Progress in spectrum-based electrochemiluminescence quantitative analysis

电化学发光(electrochemiluminescence,ECL)是一种将电化学分析和化学发光分析相结合的现代分析技术,已在多个领域得到广泛应用.ECL辐射与电化学过程密切相关,其辐射强度通常伴随电化学反应的进行而变化,导致难以采用波长扫描方式准确采集ECL光谱.以点式光电转换元件——光电倍增管(PMT)作为检测器的传统ECL分析仪通常采用摒弃色散装置的构造设计,只能开展光强型ECL分析.采用色散装置与面阵/线阵光电转换元件相结合方式构建曝光成像摄谱型ECL检测装置,发展光谱型ECL分析技术,能够推动ECL光谱学及其分析应用的研究.本文在概述ECL检测装置发展历史的基础上,综述了光谱分辨型ECL定量分析新体系、新技术和新装置的研究进展,展望了光谱分辨型ECL定量分析的前景及相关仪器技术的产业化和国产化途径.

Electrochemiluminescence(ECL)is the light emission triggered by electrochemical redox,and has been developed as an analytical technology with the combined merits of electrochemistry and chemiluminescence.ECL has been widely used in varied domains,but unfortunately its intensity usually changes along with the proceeding of electrochemical reaction,which makes it difficult to accurately determine ECL spectrum in a wavelength-scanning way with photomultiplier tube(PMT)as detector.The PMT-based traditional ECL analyzers are commonly designed with a dispersion-free architecture and can only perform ECL analysis in a total light intensity-detecting way.The development of ECL spectrum analyzer and corresponding technologies is being achieved in an alternative way by employing a grating as dispersion device and a charge-coupled device(CCD)as a detector,which can record the ECL by exposing the dispersed ECL to CCD,and figure out the corresponding spectrum with varied elements of CCD.This article briefed the developing history of ECL setups,reviewed the progress of monochromatic ECL systems,technologies and devices qualified for spectrum-resolved ECL quantitative analysis,and also prospected the development of spectrum-resolved ECL quantitative analysis as well as the industrialization and localization of ECL spectrum analyzers.