四环素和砷对斑马鱼的联合毒性及机制

Combined toxicity and underlying mechanism of tetracycline and arsenic on zebrafish

选取斑马鱼为模式生物,研究TET(四环素)和As(砷)对斑马鱼的联合毒性.结果表明,50μg/L TET和100μg/L As的联合暴露显著增加斑马鱼体内氧化应激(GPx)、炎症因子(TNF-α)和凋亡因子(C-jun)相关基因表达,造成肠道和肝脏组织更严重的损伤.50μg/L TET和100μg/L As联合暴露呈现的协同毒性效应与P-gp基因被抑制介导的As在肠道和肝脏蓄积量[(704.15±24.50)ng/g和(458.35±24.25ng/g)]显著增加有关.此外,发现肠道的通透性及微生物群落结构的变化对TET和As协同毒性无明显影响.

Zebrafish was selected as the model organism to explore the combined toxicity of TET and As on aquatic organisms.The results showed that the combined exposure of 50μg/L TET and 100μg/L As significantly increased gene expression of oxidative stress(GPx),inflammatory factors(TNF-α)and apoptosis factors(C-jun),and caused severer damage to the intestine and liver in zebrafish.The synergistic toxicity of 50μg/L TET and 100μg/L As was related to the inhibition of P-gp gene,which significantly increased the accumulation of As in intestinal tract and liver[(704.15±24.50)ng/g and(458.35±24.25ng/g)].In addition,the results of this study indicated that the changes of intestinal permeability and microbial community structure had little effect on the synergistic toxicity of TET and As.The results of this study provided basic data for the evaluation of the combined effects of TET and As.