苯并[a]蒽与肺表面活性剂混合磷脂的相互作用

Interaction between benz[a]anthracene and pulmonary surfactant mixed phospholipid monolayers

为探究多环芳烃对肺表面活性物质(PS)膜的影响,选取1,2-二棕榈酰-sn-甘油-3-磷酸胆碱(DPPC)和1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱(POPC)混合磷脂为PS模型,研究了苯并[a]蒽与DPPC/POPC混合磷脂的界面化学相互作用.结果表明,苯并[a]蒽对DPPC/POPC混合膜的相变影响显著,会削弱膜的抗形变能力、干扰膜分子的致密有序排列,这一不利影响随POPC占比增多更加明显.DPPC/POPC混合磷脂胶束会包裹苯并[a]蒽对其产生增溶作用,当DPPC浓度为200mg/L时,苯并[a]蒽的表观溶解度接近水溶液的3倍且POPC含量增加会促进胶束的分散使增溶更显著.苯并[a]蒽与DPPC/POPC混合磷脂的相互作用既抑制PS的生理功能,又会影响苯并[a]蒽的溶解迁移,进一步增加苯并[a]蒽的暴露风险.同时生理条件变化引起PS组分的改变可能导致苯并[a]蒽潜在毒性的差异,对患有相关肺部疾病的人群造成的肺损伤可能更严重.

To investigate the effect of polycyclic aromatic hydrocarbons on pulmonary surfactant(PS)monolayers,mixed phospholipids of 1,2-dipalmitoyl-sn-glycerin-3-phosphate(DPPC)and 1-palmitoyl-2-oleoyl-sn-glycerine-3-phosphatidylcholine(POPC)were selected as PS models to study the interface chemistry reaction between benz[a]anthracene and DPPC/POPC mixed phospholipids.The results showed that benz[a]anthracene can significantly affect the phase transition of DPPC/POPC mixed phospholipid monolayers and weaken the stability of the monolayers.Furthermore,benz[a]anthracene disturbed the dense and ordered arrangement of phospholipid molecules.With the increase of the proportion of POPC,the adverse effects were aggravated.DPPC/POPC mixed micelles can enclose benz[a]anthracene promoting the dissolution of benz[a]anthracene.When the concentration of DPPC is 200mg/L,the apparent solubility of benz[a]anthracene is nearly three times that of aqueous solution and the solubilization was more obvious with the increase of POPC,which was mainly due to the fact that POPC was beneficial to the dispersion of micelles.We can draw a conclusion that benz[a]anthracene can inhibit the physiological function of PS,and PS can in turn affect the dissolution and migration of benz[a]anthracene,further increase the exposure risk of benz[a]anthracene.At the same time,the change of PS components caused by physiological conditions may lead to the difference of potential toxicity of benz[a]anthracene,and the lung injury of people with related lung diseases may be more serious.