D-阿洛酮糖酶法生产的研究进展

Recent Advances in Enzymatic Production of D-Allulose

稀有单糖D-阿洛酮糖是D-果糖的C-3差向异构体,主要通过D-塔格糖3-差向异构酶或D-阿洛酮糖-3-差向异构酶对D-果糖进行异构化获得。D-阿洛酮糖不仅可以作为食品添加剂和膳食补充剂,而且具有改善胰岛素抵抗、增强抗氧化和降血糖控制等多种生理功能。因此,D-阿洛酮糖作为传统高能量糖如蔗糖、果糖等的健康替代品,具有重要的研究价值。作者综述了D-阿洛酮糖的理化性质、来源、体内代谢、生理功能、应用和最新生产技术,讨论了D-阿洛酮糖生产中存在的问题及解决方案。针对低废物生成、低能耗、高糖产量等特点,提出了一种绿色、可循环利用的D-阿洛酮糖生产工艺技术。

The rare monosaccharide D-allulose is a C-3 epimer of D-fructose,which is mainly produced through the isomerization of D-fructose by D-tagatose 3-epimerases(DTEases)or D-allulose 3-epimerases(DAEases).D-Allulose can not only be used as a food ingredient and dietary supplement,but also exhibits a variety of physiological functions and health benefits such as insulin resistance improvement,antioxidant enhancement and hypoglycemic controls.Therefore,D-allulose is of great value in development and research as a healthy alternative for conventional high-energy sugars such as sucrose and fructose.This review provides a systematic analysis of physicochemical property,existing sources,metabolism in vivo,physiological functions,applications and recent processing technologies of D-allulose.The existing problems and proposed solutions in the production of D-allulose are discussed.A green and recyclable process technology for D-allulose production is also proposed for low waste generation,low energy consumption and high conversion.