一株鸡源性沙门氏菌的基因组分析

Genome Analysis of a Chicken-derived Salmonella

为研究生鲜鸡肉中沙门氏菌的基因组信息,了解其耐药机制与致病机制,从新鲜鸡肉中分离出一株沙门氏菌FC10727,采用Illumina HiSeq 4000和PacBio对其进行全基因组测序与生物信息学分析。结果表明,FC10727基因组大小为4638420 bp,预测有4016个基因,GC含量为54.15%。通过注释eggNOG数据库,对应基因有944个,按功能可分为20类;通过比对KEGG数据库,得到2981个基因,共参与24类代谢通路;通过比对GO数据库,得到2901个基因,按基因功能可分为42类;通过比对CAZy数据库,得到96个基因,共分为5类。此外,数据库分析结果提示,该菌株共有耐药基因14个,具有β-内酰胺类药物耐药性等多种耐药机制;致病基因27个,携带系统性红斑狼疮致病基因及多种致病因子。

To explore the bioinformatic characteristics,pathogenicity and drug resistance of Salmonella in fresh chicken,a Salmonella strain FC10727 was isolated from a fresh chicken purchased from an agricultural market in Changsha City.Illumina HiSeq 4000 and PacBio were used for whole genome sequencing and bioinformatics analysis of FC10727.Its genome was found to have 4638420 base pairs,with 54.15%GC content,and was predicted to encode 4016 genes.There were 944 corresponding genes found in the eggNOG library,which can be divided into 20 categories based on their functions.In the KEGG library,there were 2981 related genes participating in a total of 24 types of metabolic pathways.In the GO library,a total of 2901 related genes were obtained,which can be divided into 42 categories.In the CAZy library,there were 96 related genes which can be divided into 5 categories.The strain has 14 drug resistance genes,with multiple drug resistance mechanisms such asβ-lactam drug resistance.And it also has 27 pathogenic genes,including systemic lupus erythematosus pathogenic genes and multiple pathogenic factors.