白细胞介素-1β、基质金属蛋白酶-7和多配体蛋白聚糖-1在子宫内膜异位症中的表达及其意义

Expression and significance of interleukin-1β,matrix metalloproteinase-7 and recombinant syndecan-1 in endometriosis

目的探讨白细胞介素-1β(IL-1β)、基质金属蛋白酶-7(MMP-7)和多配体蛋白聚糖-1(SDC-1)在子宫内膜异位症中的表达及其意义。方法随机选择2019年1月—2020年12月在宝安区中心医院住院的100例活动期子宫内膜异位症患者作为研究对象,参照腹腔镜诊断子宫内膜异位症的评分系统(r-AFS)分期法,将研究对象分为轻度组和中重度组,每组各50例,同期选取50例健康女性者作为对照组。采用酶联免疫吸附测定(ELISA)法检测并比较分析各组血清IL-1β、MMP-7和SDC-1含量,分析IL-1β、MMP-7和SDC-1表达与r-AFS评分的关系。结果中重度组的IL-1β、MMP-7和SDC-1表达水平均高于轻度组与对照组,差异有统计学意义(P<0.05);轻度组的IL-1β、MMP-7和SDC-1表达水平均高于对照组,差异有统计学意义(P<0.05)。相关性分析显示,IL-1β、MMP-7和SDC-1表达与子宫内膜异位症活动度呈显著正相关(r=0.78、0.85、0.82,P<0.05)。结论IL-1β、MMP-7和SDC-1在子宫内膜异位症中呈高表达,三者可能相互协同,促进子宫内膜异位病灶的发生和发展。

Objective To explore the expression and significance of interleukin-1β(IL-1β),matrix metalloproteinase-7(MMP-7)and recombinant syndecan-1(SDC-1)in endometriosis.Methods A total of 100 patients with active endometriosis who were hospitalized in Bao′an Central Hospital of Shenzhen City from January 2019 to December 2020 were randomly selected as the research objects.According to the revised American Fertility Society,the study subjects were divided into mild group and moderate to severe group,50 cases in each group,and 50 healthy women were selected as the control group during the same period.The enzyme-linked immunosorbent assay(ELISA)method was used to detect and compare the levels of serum IL-1β,MMP-7 and SDC-1 in each group,and the relationship between the expression of IL-1β,MMP-7 and SDC-1 and r-AFS score was analyzed.Results The expression levels of IL-1β,MMP-7 and SDC-1 in the moderate to severe group were higher than those in the mild group and the control group,and the differences were statistically significant(P<0.05).The expression levels of IL-1β,MMP-7 and SDC-1 in the mild group were higher than those in the control group,and the differences were statistically significant(P<0.05).Correlation analysis showed that the expressions of IL-1β,MMP-7 and SDC-1 were significantly positively correlated with endometriosis activity(r=0.78,0.85,0.82,P<0.05).Conclusion IL-1β,MMP-7 and SDC-1 are highly expressed in endometriosis,and the three may cooperate with each other to promote the occurrence and development of endometriosis lesions.