基于高密度SNP标记对玉米穗部相关性状的QTL定位及候选基因分析

QTL Mapping and Candidate Gene Analysis about Ear-Related Traits in Maize Based on High Density SNP Markers

以T32和齐319为亲本构建118份F2∶3家系的双亲分离群体为材料,通过对不同家系的穗部性状进行评价,结合高密度SNP标记的基因型鉴定结果,利用IciMapping4.2软件中的复合区间作图法对5个穗部相关性状(穗长、穗粗、穗行数、行粒数和秃尖长)进行QTL定位。结果表明,共检测到16个QTL,其中,控制穗长、穗粗、穗行数、行粒数和秃尖长的QTL分别为3、2、4、2和5个,单个QTL可解释2.92%~13.53%的表型变异。结合公共数据库,利用生物信息学分析筛选出4个控制穗部相关性状的候选基因Zm00001d031906、Zm00001d027721、Zm00001d002762和Zm00001d002768。

A two-parent segregating population of 118 F2∶3 lines was constructed using T32 and Qi319 as parents.Ear related traits of different families were evaluated,and genotype of each plants were identified by using high-density SNP markers.QTL mapping was conducted for 5 ear-related traits(ear length,ear diameter,ear row number,kernel number per row,ear barren tip)by using composite interval mapping method in IciMapping4.2 software.The result showed that,a total of 16 QTLs were detected,wherein 3,2,4,2 and 5 QTLs were detected to be related with ear length,ear diameter,ear row number,kernel number per row and ear barren tip,respectively.A single QTL could explain phenotypic variation of 2.92%-13.53%.Based on the public database,5 suspected key candidate genes related with spike related traits were screened by using bioinformatics analysis,which including genes of Zm00001d031906,Zm00001d02721,Zm00001d002762,Zm00001d002768.