摘要
以T32和齐319为亲本构建118份F2∶3家系的双亲分离群体为材料,通过对不同家系的穗部性状进行评价,结合高密度SNP标记的基因型鉴定结果,利用IciMapping4.2软件中的复合区间作图法对5个穗部相关性状(穗长、穗粗、穗行数、行粒数和秃尖长)进行QTL定位。结果表明,共检测到16个QTL,其中,控制穗长、穗粗、穗行数、行粒数和秃尖长的QTL分别为3、2、4、2和5个,单个QTL可解释2.92%~13.53%的表型变异。结合公共数据库,利用生物信息学分析筛选出4个控制穗部相关性状的候选基因Zm00001d031906、Zm00001d027721、Zm00001d002762和Zm00001d002768。
A two-parent segregating population of 118 F2∶3 lines was constructed using T32 and Qi319 as parents.Ear related traits of different families were evaluated,and genotype of each plants were identified by using high-density SNP markers.QTL mapping was conducted for 5 ear-related traits(ear length,ear diameter,ear row number,kernel number per row,ear barren tip)by using composite interval mapping method in IciMapping4.2 software.The result showed that,a total of 16 QTLs were detected,wherein 3,2,4,2 and 5 QTLs were detected to be related with ear length,ear diameter,ear row number,kernel number per row and ear barren tip,respectively.A single QTL could explain phenotypic variation of 2.92%-13.53%.Based on the public database,5 suspected key candidate genes related with spike related traits were screened by using bioinformatics analysis,which including genes of Zm00001d031906,Zm00001d02721,Zm00001d002762,Zm00001d002768.
作者
赵强
陈柔屹
王安贵
郭向阳
刘鹏飞
祝云芳
吴迅
陈泽辉
ZHAO Qiang;CHEN Rou-yi;WANG An-gui;GUO Xiang-yang;LIU Peng-fei;ZHU Yun-fang;WU Xun;CHEN Ze-hui(Institute of Dry Grain Research,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)
出处
《玉米科学》
CAS
CSCD
北大核心
2021年第3期36-41,共6页
Journal of Maize Sciences
基金
国家“七大作物育种”专项(2016YFD0101206-4,2018YFD0100104)
国家自然科学基金项目(31760387)
黔科合基础〔2020〕1Z019
黔科合基础〔2017〕1413
黔科合支撑项目〔2017〕2507
黔科合支撑项目〔2018〕2296
黔科合人才项目〔(2018〕5629)
黔科中引地项目〔2018〕4003
黔人项目资助合同〔2015〕08号。