摘要
目的:建立并验证一种定性定量检测人全血中乙醇、甲醇、异丙醇、丙酮的方法,并将其应用于临床检测。方法:采用双流路顶空气相仪测定,色谱柱为Rtx-BAC2(30 m,0.32 mm,1.2μm)、SH-Rtx-BAC1(30 m,0.32 mm,1.8μm),以氮气为载气,色谱柱流量为4 mL/min,分流比为30∶1,柱温箱温度为40℃,FID温度为250℃。结果:本方法乙醇、甲醇、异丙醇、丙酮在0.0625~8 mg·mL^(-1)内线性关系良好,批内相对标准偏差(RSD)和相对误差(RE)分别为2.31%~10.72%和-9.50%~8.27%,批间RSD和RE分别为1.50%~10.86%和-9.09%~9.60%,在多种储存条件下,全血样本稳定性好。结论:本方法检测速度快、专属性强、准确度高、重复性好,本文设计了合理的标准曲线范围和质控浓度,可覆盖临床中毒检测范围。
Objective:To establish and validate a method for qualitative and quantitative detection of ethanol,methanol,isopropanol,acetone in human whole blood which can be applied to clinical test.Methods:Double flow gas chromatography was used for determination.The chromatographic column was Rtx-BAC2(30 m,0.32 mm,1.2μm)and SH-Rtx-BAC1(30 m,0.32 mm,1.8μm).The carrier gas was nitrogen.The column flow was 4 mL/min,and the split ratio was 30:1.The oven temperature was 40℃,and the temperature of FID was 250℃.Results:The linear ranges of ethanol,methanol,isopropanol and acetone in this method were 0.0625-8 mg·mL^(-1).The intra-assay relative standard deviation(RSD)and relative error(RE)were 2.31%-10.72% and -9.50%-8.27%.The inter-assay RSD and RE were 1.50%-10.86% and -9.09%-9.60%.Whole blood samples were stable under various storage conditions.Conclusion:The method is fast,specific,accurate and repeatable.The standard linear range and quality control concentration are reasonably designed,which can cover the ranges of clinical toxic concentration.
作者
高宇阳
李春林
毕重文
高卫真
GAO Yu-yang;LI Chun-lin;BI Chong-wen;GAO Wei-zhen(Department of Clinical Pharmacy,College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Department of Pharmacy,General Hospital,Tianjin Medical University,Tianjin 300052,China)
出处
《天津医科大学学报》
2021年第4期409-412,共4页
Journal of Tianjin Medical University
基金
天津市自然科学基金(18JCQNJC83200)。
关键词
顶空气相法
有机溶剂
全血
headspace gas chromatography
organic solvent
whole blood