双流路顶空气相法检测人全血中乙醇、甲醇、异丙醇、丙酮及其临床应用

Determination of ethanol,methanol,isopropanol and acetone in human whole blood by double flow headspace air phase and its clinical application

目的:建立并验证一种定性定量检测人全血中乙醇、甲醇、异丙醇、丙酮的方法,并将其应用于临床检测。方法:采用双流路顶空气相仪测定,色谱柱为Rtx-BAC2(30 m,0.32 mm,1.2μm)、SH-Rtx-BAC1(30 m,0.32 mm,1.8μm),以氮气为载气,色谱柱流量为4 mL/min,分流比为30∶1,柱温箱温度为40℃,FID温度为250℃。结果:本方法乙醇、甲醇、异丙醇、丙酮在0.0625~8 mg·mL^(-1)内线性关系良好,批内相对标准偏差(RSD)和相对误差(RE)分别为2.31%~10.72%和-9.50%~8.27%,批间RSD和RE分别为1.50%~10.86%和-9.09%~9.60%,在多种储存条件下,全血样本稳定性好。结论:本方法检测速度快、专属性强、准确度高、重复性好,本文设计了合理的标准曲线范围和质控浓度,可覆盖临床中毒检测范围。

Objective:To establish and validate a method for qualitative and quantitative detection of ethanol,methanol,isopropanol,acetone in human whole blood which can be applied to clinical test.Methods:Double flow gas chromatography was used for determination.The chromatographic column was Rtx-BAC2(30 m,0.32 mm,1.2μm)and SH-Rtx-BAC1(30 m,0.32 mm,1.8μm).The carrier gas was nitrogen.The column flow was 4 mL/min,and the split ratio was 30:1.The oven temperature was 40℃,and the temperature of FID was 250℃.Results:The linear ranges of ethanol,methanol,isopropanol and acetone in this method were 0.0625-8 mg·mL^(-1).The intra-assay relative standard deviation(RSD)and relative error(RE)were 2.31%-10.72% and -9.50%-8.27%.The inter-assay RSD and RE were 1.50%-10.86% and -9.09%-9.60%.Whole blood samples were stable under various storage conditions.Conclusion:The method is fast,specific,accurate and repeatable.The standard linear range and quality control concentration are reasonably designed,which can cover the ranges of clinical toxic concentration.