向日葵抗列当基因Or5的抗性遗传及分子标记筛选

Sunflower Broomrape Resistance Inheritance Analysis and Screening of Molecular Markers

以向日葵高感列当E的自交系09110-21264162A为母本,与抗病自交系J-09R进行杂交构建F 2群体,分析了向日葵抗列当E的抗性遗传规律,并且采用SSR技术在亲本和F 2代群体中筛选与抗列当基因Or5连锁的分子标记。结果表明:F 2群体的抗感比符合3∶1的分离规律,筛选出与抗列当基因Or5连锁的1个分子标记ORS1036。经F 2分离群体验证,该标记的表型鉴定结果与分子检测结果的一致性为94%。将该标记对84份自交系进行筛选,获得了15份可能携带Or5基因的抗列当资源,为抗性育种提供宝贵资源。

The study was carried out by cross-breeding between sunflower broomrape E susceptible inbred line 09110-21264162A and the resistant inbred line J-09R as parents to construct the F 2 population.The parents,F 1 and F 2 generations were inculated with broomrape and the inheritance of resistance to the plant was analyzed according to the results of phenotype observation.The linked marker was screened with two parents and a F 2 population by SSR technology.The results revealed that the identification result of the individual plant of the F 2 population was in line with the anti-infection ratio of 1R:3S.The Molecular Marker,ORS1036,which was linked to Or5,was screened from F 2 population and the parents by SSR technique.The results of phenotypic identification was 94%consistent with the molecular detection.This marker ORS1036 was used to screen 84 inbred lines and 15 materials which contained the marker were obtained,which could provide valuable resources for resistance breeding.