HPLC法同时测定宝咳宁颗粒中8个成分的含量

Simultaneous Determination of Eight Constituents in Baokening Granules by HPLC

目的:建立同时测定宝咳宁中苦杏仁苷、甘草苷、甘草酸铵、白花前胡甲素、白花前胡乙素、黄芩苷、橙皮苷和新橙皮苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为Agilent ZORBAX SB-C_(18)柱(250 mm×4.6 mm,5μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速1.0 ml·min^(-1),检测波长为210 nm(苦杏仁苷)、237 nm(甘草苷、甘草酸铵)、321 nm(白花前胡甲素、白花前胡乙素)、280 nm(黄芩苷、橙皮苷、新橙皮苷),柱温30℃,进样量20μl。结果:苦杏仁苷、甘草苷、甘草酸铵、白花前胡甲素、白花前胡乙素、黄芩苷、橙皮苷和新橙皮苷检测质量浓度的线性范围分别为20.47327.52μg·ml^(-1),3.1350.05μg·ml^(-1),1.5424.67μg·ml^(-1),11.26180.19μg·ml^(-1),6.75108.00μg·ml^(-1),7.43118.88μg·ml^(-1),3.1750.69μg·ml^(-1),4.9178.59μg·ml^(-1)(r为0.99930.9999);平均加样回收率分别为101.2%,101.0%,100.8%,98.6%,101.3%,100.0%,99.2%,100.8%(RSD<2.0%,n=6)。结论:该方法可用于宝咳宁颗粒的质量评价。

Objective:To develop a method for the simultaneous determination of amygdalin,liquiritin,ammonium glycyrrhizinate,praeruptorin A,praeruptorin B,baicalin,hesperidin and neohesperidin in Baokening granules by HPLC.Methods:The analysis was carried on an Agilent ZORBAX SB-C_(18) column(250 mm×4.6 mm,5μm)with the mobile phase consisting of acetonitrile-0.2%phosphoric acid in a gradient elution mode at the flow rate of 1.0 ml·min^(-1).The detection wavelengths were set at 210 nm for amygdalin,237 nm for liquiritin and ammonium,321 nm for praeruptorin A and praeruptorin B,280 nm for baicalin,hesperidin and neohesperidin.The column temperature was set at 30℃and the injection volume was 20μl.Results:The linear range was 20.47-327.52μg·ml^(-1),3.13-50.05μg·ml^(-1),1.54-24.67μg·ml^(-1),11.26-180.19μg·ml^(-1),6.75-108.00μg·ml^(-1),7.43-118.88μg·ml^(-1),3.17-50.69μg·ml^(-1) and 4.91-78.59μg·ml^(-1) for amygdalin,liquiritin,ammonium glycyrrhizinate,praeruptorin A,praeruptorin B,baicalin,hesperidin and neohesperidin(r=0.9993-0.9999),respectively.The average recovery was 101.2%,101.0%,100.8%,98.6%,101.3%,100.0%,99.2%and 100.8%(RSD<2.0%,n=6),respectively.Conclusion:The established method can be used for the quality control of Baokening granules.