HDAC4 knockdown alleviates denervation-induced muscle atrophy by inhibiting myogenin-dependent atrogene activation

Denervation can lead to progressive skeletal muscle atrophy.At present,there is no effective treatment for muscle atrophy.Histone deacetylase 4(HDAC4)has recently been found to be closely related to muscle atrophy,but the underlying mechanism of HDAC4 in denervation-induced muscle atrophy have not been described clearly.In this study,HDAC4 inhibition can effectively delay denervation-induced muscle atrophy,reduce the expression of muscle specific E3 ubiquitin ligase and autophagy related proteins,alleviate muscle fiber type transition and enhance the expression of SIRT1 and PGC1a.Transcriptome sequencing and bioinformatics analysis was performed and suggested that HDAC4 may be involved in denervation induced muscle atrophy by regulating response to denervation involved in regulation of muscle adaptation,cell division,cell cycle,apoptotic process,skeletal muscle atrophy,and cell differentiation.