The role of neuronal Fc-epsilon receptor 1 in itch of allergic conjunctivitis rat model

Itch has been an increasingly concerned clinical problem as it has caused needless suffering of people's life.However,current therapeutics targeting the immune system sometimes fail to achieve satisfactory results.We hereby explored the role of neuronal Fc-epsilon receptor I(FceRI)in itch of allergic conjunctivitis rat model.After establishment of allergic conjunctivitis model in experimental groups of rats by ovalbumin as allergen,we observed scratch behavior of both experimental groups and control groups,with scratching eyes by hind lim bs regarded as itch-related behavior.The allergic wild type rats showed significantly elevation of scratch behavior compared to the naive rats,as well as the allergic rats with neuron conditional knockout of FceRI.Meanwhile,we detected the expression of FceRI and pSyk,which is the main downstream molecule of FceRI,in neurons in the trigeminal ganglion(TG)by Western blotting and immunohistochemistry,which showed up-regulation in allergic rats.