Research on downstream target genes of transcription factor Brn4

Our previous studies indicated that transcription factor Brn4 could promote neuronal differentiation of hippocampal neural stem cells,but its molecular mechanism remains unknown.In this research,we used high-throughput sequencing methods,RNA-seq and ChIP-seq,to detect the changes of gene expression level in Brn4 overexpression cells.The RNA-seq results were screened based on the difference of more than 1.5 times and intersected with ChIP-seq.The 16 genes in the intersection were considered as candidate genes.