The function of adipose-derived Schwann-like cells on sciatic nerve regenerati on and repair

Schwann cells(SCs)combined with acellular nerve allografts(ANAs)effectively promote the regeneration and repair of peripheral nerves.However,autologous SCs are difficult to obtain and expand to sufficient numbers in a short duration due to limitations in tissue availability,morbidity of the donor site and the need to sacrifice one or more functioning nerves,resulting in loss of sensation.Therefore,adipose derived stem cells(ADSCs)were introduced into SCs.Then Schwann-like cells combined with ANAs to bridge the missing sciatic nerves.In this study,the rats were randomly divided into five groups:Control group,Model group,ADSC group,SC-L group and DMEM group.Twelve weeks later postsurgery,behavioral function tests and molecular biological techniques were used to evaluate the function of regenerated nerves after sciatic nerve injury.The behavioral experiment analysis showed that the SFI,NCV and WA in SC-L group were considerably higher than those in ADSC,DMEM and model groups.