Parabiotic strategy alleviate Alzheimer s neuropathology through peripheral cireulation

To investigate the impact of systemic circulation on Alzheimer's disease(AD)like neuropathology and the recruitment of peripheral immune cells to the brain in the pathogenic process of AD.We performed parabiotic surgeries between C57BL/6(B6)congenic APP/PS1 transgenic(Tg)mice(ParaAPP/PS1)and sex/age matched B6 congenic green-fluorescent-protein(GFP;βactin promoter)Tg mice(ParaWT)at3 to6 months.The mice remained parabiosed until 7 to 10 months,followed by dissection for tissue collection and analyses.