DMT1 overexpression exacerbates Aβoligomer-induced cell damage

This study aims to reveal the role of metal ion transporter DMT1 in neurotoxicity induced by Aβ142 oligomers.We exposed SH-SY5Y cells(S-DMT1)stably overexpressing the DMT1 gene and SH-SY5Y cells(SGFP)overexpressing empty green fluorescent protein to AB.42 oligomers.CCK8 and Hoechst 33258 were used to detect the cell death rate and apoptotic rate.W estern blotting was used to detect the levels of apoptotic proteins caspase-3,Bax and Bcl-2.The results of CCK8 experiments showed that compared with SGFP cells,the number of viable cells in S-DMT1 cells after Aβ42 oligomer treatment was significantly reduced.Heochst33258 staining results showed that the rate of nuclear shrinkage in S-DMT1 cells treated with Aβ142 oligomer was significantly higher than that in SGFP group.Moreover,the levels of caspase-3 and Bax proteins in S-DMT1 cells were higher than those in SGFP group,while BcI-2 decreased after Aβ42 oligomers.DMT1 overexpression increases the toxic effects of Aβ42 oligomers by promoting apoptosis.4.Regulation of"P35/P25-CDK5-Tau"signaling pathway in hippocampi and prefrontal cortex of AD rats by electroacupuncture.