P16 deletion ameliorates damage of intestinal epithelial barrier and microbial dysbiosis in a stress-induced premature senescence model of Bmi-1 deficiency

To investigate whether Bmi-l deficiency lead to intestinal epithelial barrier destruction and microbiota dysfunction and whether p16 deletion could reverse the damage of intestinal epithelial barrier and microbial dysbiosis.Bmi-l and pl6 double-knockout(Bmi-17 pl6/)mice were generated.The barrier structure and function of epithelium,the species,abundance,and function of microflora,and proinflammation of macrophages in intestine were compared with Bmi-l/and wild-type(WT)mice.Bone marrow derived macrophages were infected with fecal mixed bacteria for observing proinflammation.