Hydroxysafflor yellow A promotes apoptosis via blocking autophagic flux in liver cancer

Hydroxysafflor yellow A(HSYA)extracted from the herb Cathartics tinctorius L.negatively regulates liver cancer growth.However,the exact mechanism of HSYA action in liver cancer remains largely unknown.In this study,HSY A inhibited liver cancer cell growth in vivo and in vitro,evidenced by cell proliferation inhibition detected by CCK8,numerous apoptotic cells shown by flow cytometry assay,and expression of apoptosis-related proteins determined by Western blot.