SKL promotes patency and endothelialization of small-caliber vascular grafts in rabbit carotid artery model

The Klotho.(KL)gene is related to aging.In this study,SKL(secreted KL)and heparin were cross:linked to the acellular small intestinal submucosa(SIS).Based on this,small-diameter tissue engineered blood vessels(TEVs)were constructed.The role of SKL on improving the patency of TEVs through promoting cell adherence was investigated.The SIS membrane was cross linked with heparin and SKL,respectively,to prepare heparin group and SKL group artificial blood vessels.The CCK8 assay showed that SKL treatment promoted endothelial cells proliferation.Western blot showed that SKL upregulated the levels of FAK and RHOA.