hiPSC-derived NSCs effectively promote the functional recovery of acute spinal cord injury in mice

Spinal cord injury(SCI)is a common disease that results in motor and sensory disorders and even lifelong paralysis..The transplantation of stem cells,such as embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),mesenchymal stem cells(MSCs),or subsequently generated stem/progenitor cells,was predicted to be a promising treatment for SCI.In this study,we aimed to investigate effect of human iPSC-derived neural stem cells(hiPSC-NSCs)and umbilical cord-derived MSCs(huMSCs)in a mouse model of acute SCI.Acute SCI mice model was established and were randomly treated as phosphate-buffered saline(PBS)(control group),repaired with 1×10^(5)hiPSC-NSCs(NSC group),and 1×10^(5)huMSCs(MSC group),respectively,in a total of 54 mice.Hind limb motor function was evaluated in open-field tests using the Basso Mouse Scale(BMS)at days post operation(dpo)1,3,5,and 7 after spinal cord injury,and weekly thereafter.Spinal cord and serum samples were harvested at dpo 7,14,and 21.