Establishment of amyotrophic lateral sclerosis induced pluripotent stem cells and neuronal differentiation

Amyotrophic lateral sclerosis(ALS)is a motor neuron disease that seriously threatens the health of patients.The mechanism is unclear.Most ALS disease models cannot accurately reflect the pathological characteristics of ALS.Induced pluripotent stem cells(iPSC)can best mimic lesions and is used to reveal the mechanism of ALS and develop therapeutic drugs.In the process of iPSC reprogramming,the selection of somatic cells and the optimization of conditions are critical to the progress of iPSC culture.Skin biopsies were used in this study to optimize the isolation and culture method of fibroblasts,and established a optimized somatic cell culture process with high tissue utilization rate,short culture period and high success rate.