3株不同血清型沙门菌感染ICR小鼠模型的建立

Establishment of ICR mouse infection models with three different serotypes of Salmonella

旨在建立3株不同血清型沙门菌的小鼠感染模型,为抗沙门菌疫苗或药物的研发提供动物评价平台。分别用鸡白痢沙门菌、肠炎沙门菌和鼠伤寒沙门菌腹腔注射4~6周龄ICR雌鼠,根据不同剂量下小鼠的生存曲线来确定最适感染剂量。小鼠按最适感染剂量攻毒后20 h处死,测定脾脏、肝脏、空肠、回肠和盲肠部位细菌载量,并对脾脏和肠道进行病理学观察。鸡白痢沙门菌、肠炎沙门菌和鼠伤寒沙门菌对ICR小鼠腹腔注射的最适剂量分别为5.6×10^(8)、3.2×10^(7)和2.5×10_(6) CFU/只。鸡白痢沙门菌主要定殖小鼠的肠道,而另外2种血清型沙门菌定殖的脏器更为广泛,可引起肝脏和脾脏病变。本研究成功建立了3株不同血清型沙门菌的ICR小鼠感染模型,为沙门菌疫苗和药物的开发提供了小鼠评价模型。

This study was aimed to establish mouse infection models of three different serotypes of Salmonella, and to provide an animal evaluation platform for the development of Salmonella-resistant vaccines or drugs. 4-6 weeks old female ICR mice were injected intraperitoneally with Salmonella Pullorum, Salmonella Enteritidis and Salmonella Typhimurium, respectively. Then, the optimal infection dose was determined according to the survival curve of the mice given different doses of the bacteria. Next, the mice were challenged with the optimal infection dose of the bacteria and were killed 20 hours later to Measure the bacterial load in the spleen, liver, jejunum, ileum and cecum of the rodents and to observe pathological features in the spleen and intestines. The results showed that the optimal doses of Salmonella Pullorum, Salmonella Enteritidis and Salmonella Typhimurium for intraperitoneal injection in ICR mice were 5.6×10^(8), 3.2×10^(7) and 2.5×10^(6) CFU per mouse, respectively. Salmonella Pullorum preferred to colonize in the intestines of the mice. Salmonella Enteritidis and Salmonella Typhimurium colonized more extensively, and might cause lesion in the liver and spleen after infection. This study successfully established mouse infection models for 3 different serotypes of Salmonella, and provided a potential model for development of vaccines and drugs against Salmonella.