烟气暴露30天对大鼠心脏基因表达谱的影响

Effects of 30-day smoke exposure on gene expression profile in hearts of rats

目的:利用基因芯片技术研究烟气暴露30 d对大鼠心脏基因表达的影响。方法:6只雄性SD大鼠分为对照组和烟气暴露组。烟气暴露组经主流烟气暴露,对照组不进行干预,30 d后取大鼠心脏,应用基因芯片技术进行差异表达基因筛选,利用基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库对差异基因进行生物信息学分析。结果:与对照组比较,烟气暴露组筛选出差异表达2倍以上的基因86个,其中上调表达60个,下调表达26个。差异表达基因富集于292种生物过程,72种细胞成分和96类分子功能以及61个信号通路。主要涉及促分裂原活化蛋白激酶结合、α-βT细胞受体复合物、细胞外渗的正调节、络氨酸代谢等相关基因通路。结论:烟气暴露30 d所致大鼠心脏的异常表达基因与信号通路主要涉及分子结合、细胞周期、信号转导等多个方面。

OBJECTIVE:To study effects of smoke exposure on gene expression in hearts of rats using gene chip technology.METHODS:Six male SD rats were divided into two groups:control(n=3)and smoke exposure(n=3).The exposure group were exposed to mainstream smoke for 30 days.Rat hearts were removed and differentially expressed genes were screened using gene chip technology.Differentially-expressed genes were analyzed using gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)databases.RESULTS:Compared with the control group,86 genes with differential expression of more than 2 times were screened out in the smoke exposure group,among which 60 genes were up-regulated and 26 genes were down-regulated.Differentially expressed genes were enriched in 292 biological processes:72 cellular components,96 molecular functions and 61 signaling pathways.These processes were involved in mitogenactivated protein kinase binding,alpha-beta T cell receptor complex,positive regulation of cellular extravasation,and tyrosine metabolism.CONCLUSION:After exposure to smoke for 30 days,differentially expressed genes and signaling pathways in the hearts of rats were mainly involved in molecular binding,cell cycle control and signal transduction,etc.