中国人群中miRNA-21对肺癌诊断价值的Meta分析

Diagnostic value of miRNA-21 for lung cancer in Chinese population:a Meta analysis

目的通过Meta系统分析评价中国人群中微小RNA-21(miRNA-21)对肺癌的诊断价值。方法全面检索万方、CNKI、维普、PubMed、Embase数据库,日期从建库至2020年10月,筛选相关文献提取数据,采用QUADAS-2标准评估文献质量,再对提取数据行初步合并,以判定异质性和诊断效能,并通过回归分析、敏感性分析及亚组分析探讨异质性来源,最后以漏斗图评估是否存在发表偏倚。结果共纳入11篇文献,其中病例组1113例,对照组1182例。合并灵敏度和特异度分别为0.76(95%CI:0.73~0.78)和0.75(95%CI:0.72~0.77),阳性似然比和阴性似然比分别为2.85(95%CI:2.54~3.20)和0.33(95%CI:0.28~0.37),诊断比值比(DOR)为9.16(95%CI:7.11~11.80),综合受试者工作特征(SROC)曲线的曲线下面积(AUC)为0.82。样本类型、内参类型及探针类型均可能是异质性来源。亚组分析显示,筛查组织样本、血清样本和SYBR探针时,miRNA-21对肺癌具有很高的诊断价值(DOR=10.61、9.46、9.71),而内参为U6的诊断价值较低(DOR=6.74)。结论中国人群中miRNA-21对肺癌的诊断价值较高,有较高的灵敏度和特异度,可作为一项提高肺癌诊断水平的良好指标。

Objective To evaluate the diagnostic value of microRNA-21(miRNA-21)in lung cancer in Chinese population by Meta-analysis.Method Wanfang,CNKI,VIP,PubMed,Embase databases were comprehensively searched from the date of establishment to October 2020.The relevant literatures were screened to extracted data.The quality of literatures was evaluated by QUADAS-2 standard,then the extracted data was preliminarily combined to determine the heterogeneity and diagnostic efficacy.The sources of heterogeneity were explored by regression analysis,sensitivity analysis and subgroup analysis.Finally,funnel plot was used to evaluate the publication bias.Result A total of 11 studies involving 1113 patients in the case group and 1182 patients in the control group.The combined sensitivity and specificity were respectively 0.76(95%CI:0.73-0.78)and 0.75(95%CI:0.72-0.77),the positive likelihood ratio and negative likelihood ratio were respectively 2.85(95%CI:2.54-3.20)and 0.33(95%CI:0.28-0.37),the diagnostic odds radio(DOR)was 9.16(95%CI:7.11-11.80),AUC was 0.82.The sample type,internal parameter and probe type may be the source of heterogeneity.The results of subgroup analysis showed that miRNA-21 had a high diagnostic value for lung cancer(DOR=10.61,9.46,9.71)when screening tissue samples,serum samples and SYBR probes for lung cancer,while the diagnostic value of internal reference U6 was lower(DOR=6.74).Conclusion miRNA-21 has high diagnostic value,sensitivity and specificity for lung cancer in Chinese population,which can be used as a good indicator to improve the diagnostic level of lung cancer.