应用优化的3T3细胞中性红摄取试验评价6种化妆品的光毒性

Evaluation of Phototoxicity of 6 Cosmetic Products by Optimized 3T3 Cell Neutral Red Uptake Test

目的建立一种针对化妆品的体外光毒性检测方法。方法选取6批市售化妆品样品,通过摸索样品的细胞毒性,确定光毒浓度区间,设立样品加阳性对照的参比物,再进行光毒性评价,通过Phototox软件分析结果。结果6种市售化妆品光刺激因子≤2,表明均无潜在光毒性。建立适合于检测化妆品的样品阳性对照,且与阳性对照结果均表现强光毒性。结论该方法初步具备对化妆品光毒性评价的可行性,适合于中国国情的体外方法标准。

OBJECTIVE To establish an in vitro phototoxicity detection method for cosmetic products.METHODS Six batches of cosmetic samples sold in the market were selected.The phototoxicity concentration range was determined by exploring the cytotoxicity of the samples.Reference substances including samples and positive controls were set up and the phototoxicity was evaluated.The results were analyzed by Phototox software.RESULTS The photo irritation factor of 6 kinds of cosmetics sold in the market was≤2,which indicated that all of them had no potential phototoxicity.The sample positive control of samples suitable for the detection of products was established.The results of sample positive control and positive control showed strong phototoxicity.CONCLUSION This method has the feasibility of evaluating the phototoxicity of cosmetics and innovates the in vitro method standard which is suitable for the situation of our country.