2种藏紫草体外抗氧化及抗菌活性比较

Comparision on the Antioxidant Activity and Antibacterial Activity of Two Origins of Radix Onosmatis

目的比较长花滇紫草和团花滇紫草75%乙醇提取物的体外抗氧化及抗菌活性。方法采用ABTS法、FRAP法、邻苯三酚自氧化法测定体外抗氧化能力,并采用肉汤连续稀释法初步考察了2种藏紫草体提取物体外对15种不同菌株的抗菌作用。结果不同浓度藏紫草的75%乙醇提取物对ABTS自由基和超氧阴离子都有较强的清除能力,与BHT阳性药相当或略高于BHT;总还原力随取物浓度增加而增强;2种藏紫草的提取物对革兰阳性菌有一定的抗菌活性,但对肠杆菌科菌株及铜绿假单胞菌均未见抗菌作用。结论2种来源的藏紫草均具有一定的体外抗氧化和抗菌作用。

OBJECTIVE To compare the antioxidant activity and antibacterial activity in vitro of 75%ethanol extracts from Onosma glomeratum and Onosma hookeri..METHODS Antioxidant activity of O.glomeratum and O.hookeri in vitro was measured as ABTS method,ferric reducing ability of plasma(FRAP)method and pyrograllol autoxidation method,while the antibacterial activities on 15 different strains in vitro were studied by broth dilution method.RESULTS The 75%ethanol extracts of Radix Onosmatis had a certain scavenging effect on ABTS free radical and superoxide anion free radical,the effect was equivalent to or slightly better than BHT;the total reducing power were becoming stronger with the increasing of concentration.Two extracts of Radix Onosmatis had certain antibacterial activity to gram-positive bacteria,but had no antibacterial activity to enterobacteriaceae strains and Pseudomonas aeruginosa.CONCLUSION Both of two sources of Radix Onosmatis have some antioxidant and antibacterial effects in vitro.