不同模拟体液对硼硅酸盐生物活性玻璃基骨水泥矿化性能的影响

Effect of Different Simulated Body Fluid on Mineralization of Borosilicate Bioactive Glass-based Bone Cement

硼硅酸盐生物活性玻璃基(Borosilicate bioactive glass-based,BBG)骨水泥由于其优异的生物活性和生物降解性,在治疗骨质疏松性骨折以及骨肿瘤、骨创伤、骨髓炎等疾病方面具有重要的应用前景,受到人们的广泛关注。为进一步了解氨基酸对其植入生物体内后的矿化影响,本研究在常规的SBF溶液中添加了不同种类及浓度的氨基酸物质,重点研究对植入体表面形貌的影响。同时为在矿化过程中同步形成白磷钙矿(Whitlockite,WH)和羟基磷灰石(hydroxyapatite,HA),调整了SBF溶液的温度以及酸碱度和Mg^(2+)浓度,研究了不同SBF溶液中BBG骨水泥表面形成的矿化产物。研究结果表明,不同的氨基酸及浓度的变化对矿化产物的影响有较大差异,天冬氨酸和赖氨酸的浓度变化影响矿物的长径比,而甘氨酸对矿物形貌的影响较小。将硼硅酸盐生物活性玻璃压片放置在70℃下的高Mg^(2+)浓度的酸性(pH=3.5)SBF溶液中浸泡一定时间后,能够获得HA/WH的复相矿物。

Borosilicate bioactive glass-based(BBG)bone cement has important application prospects in the treatment of osteoporotic fractures,bone tumors,bone trauma,osteomyelitis,and other diseases due to its excellent bioactivity and biodegradability.To further understand the factors affecting its mineralization ability after implantation into human body,three kinds of amino acid were added into the conventional simulated body fluids(SBF)solution.Furthermore,to get whitlockite(WH)and hydroxyapatite(HA)simultaneously during the mineralization process,the environmental temperature,pH and Mg^(2+) concentration of SBF were adjusted.The mineralized products of BBG bone cement after being immersed in different SBF solutions were characterized.It is found that the effects of amino acids on mineralization products are quite different,of which aspartic acid and lysine affect the aspect ratio of the mineral,but glycine does not affect the morphology of the mineral.After the borosilicate bioactive glass tablet being immersed in an acidic(pH 3.5)SBF solution with a high Mg^(2+)content at 70℃for a certain period,HA/WH complex mineral can be obtained.