Li_(2)Ca_(2)Si_(2)O_(7)生物陶瓷的矿化活性研究

Mineralization Activity of Li_(2)Ca_(2)Si_(2)O_(7) Bioceramics

如何有效治疗牙周炎并实现受损牙周骨组织再生,一直是牙周疾病治疗中具有挑战性的问题,而矿化是牙周正常发育和功能中关键因素之一。本研究旨在探讨硅酸钙锂(Li_(2)Ca_(2)Si_(2)O_(7))生物陶瓷对人牙周膜成纤维细胞增殖、矿化的影响及用于牙周骨组织再生的可能性。采用溶胶–凝胶法制备合成了Li_(2)Ca_(2)Si_(2)O_(7)陶瓷粉体。通过体外模拟体液浸泡,发现Li_(2)Ca_(2)Si_(2)O_(7)粉体具有良好的羟基磷灰石矿化能力。生物学结果表明:Li_(2)Ca_(2)Si_(2)O_(7)粉体的浸提液在3.125~25 mg/m L浓度范围内能显著促进HPLFs的增殖,低浓度(6.25 mg/m L)时可显著诱导HPLFs细胞体外矿化(p<0.05)。Li_(2)Ca_(2)Si_(2)O_(7)粉体具有促进人牙周膜成纤维细胞增殖和矿化能力,有望作为牙周骨组织再生修复的生物活性材料。

How to effectively treat periodontitis and achieve regeneration of damaged periodontal tissue has always been a challenging problem in the treatment of periodontal diseases,and mineralization is one of the key factors in the normal periodontal development and function.The aim of this study was to investigate the effects of Li_(2)Ca_(2)Si_(2)O_(7) bioceramics on proliferation and mineralization of human periodontal ligament fibroblasts(HPLFs)and possibility of its application in periodontal tissue regeneration.Li_(2)Ca_(2)Si_(2)O_(7) ceramic powder was synthesized by Sol-Gel method.It was found that Li_(2)Ca_(2)Si_(2)O_(7) powder had good ability of hydroxyapatite mineralization by immersing in simulated body fluid.The in vitro biological results showed that Li_(2)Ca_(2)Si_(2)O_(7) powder extract could significantly promote proliferation of HPLFs in the concentration range of 3.125~25 mg/mL,and at low concentration(6.25 mg/mL),could significantly induce mineralization of HPLFs cells(p<0.05).All these experimental results reveal that the new Li_(2)Ca_(2)Si_(2)O_(7) powder can promote proliferation and mineralization of HPLFs,and it may be a promising bioactive material for periodontal tissue regeneration.