藏东南产区葡萄和根际土壤细菌群落多样性

Bacterial community diversity of grape and rhizosphere soil of grapevine plants grown in southeastern Tibet

为了分析藏东南产区葡萄及根际土壤细菌群落结构及多样性,采用高通量测序技术对西藏2个种源地葡萄和根际土壤细菌群落结构和多样性进行了系统分析。结果表明,2个种源地葡萄和土壤样品共获得366720条有效序列,2655个可操作分类单元(operational taxonomic units,OTUs),隶属32门、713属,不同样品间细菌群落存在一定的共性和差异且土壤细菌种类最多,多样性最高。不同样品中的优势菌门均为变形菌门(Proteobacteria)和放线菌门(Actinobacteria),其相对丰度分别为21.31%~69.08%和21.41%~44.60%;林芝葡萄(LP)中的优势属是伯克氏菌属(Burkholderia)(27.60%)和脆弱拟杆菌(Muribaculaceae)(5.34%),而芒康葡萄(MP)中的细菌以葡萄糖杆菌属(Gluconobacter)(22.53%)、冰冻小杆菌属(Frigoribacterium)(14.14%)和泛菌属(Pantoea)(13.52%)为主;林芝根际土壤(LS)中的优势属是节杆菌属(Arthrobacter)(11.49%)、肠杆菌科(Enterobacteriaceae)(7.43%)和类诺卡氏菌属(Nocardioides)(5.15%),而芒康根际土壤(MS)中的优势属是Gaiella(5.17%)和Llumatobacteraceae(4.43%),不同样品中优势菌群差异较大。主成分分析表明,2个种源地间葡萄样品中细菌群落组成相似,而土壤中细菌群落组成差异较大。该研究为西藏设施葡萄种植和酿酒细菌的筛选与利用提供了理论依据。

To investigate the bacterial community and diversity of grapevine plants grown in southeastern Tibet,the bacterial community and diversity of grape and rhizosphere soil of different provenances in southeastern Tibet were analyzed by high-throughput sequencing.The results showed that a total of 366720 valid sequences and 2655 operational taxonomic units(OTUs)were obtained across all libraries,representing 713 genera in 32 bacterial phyla.There were similarities and differences in bacterial communities among different samples,and the rhizosphere soil showed the highest number and diversity of bacteria.The Proteobacteria and Actinobacteria were the predominant bacterial phyla in all samples,accounting for 21.31%-69.08%and 21.41%-44.60%,respectively.Burkholderia(27.60%)and norank_f_Muribaculaceae(5.34%)were the predominant genera in Linzhi grape,while Gluconobacter(22.53%),Frigoribacterium(14.14%)and Pantoea(13.52%)were the predominant genera in Mangkang grape.Arthrobacter(11.49%)、unclassified_Enterobacteriaceae(7.43%)and Nocardioides(5.15%)were the predominant genera in Linzhi soil,while Gaiella(5.17%)and norank_f_Llumatobacteraceae(4.43%)were the predominant genera in Mangkang soil.The predominant bacteria were significantly different in different samples.Principal coordinates analysis(PCoA)showed that the bacterial community in grape samples was similar,however,the bacterial community of rhizosphere soil was significantly different.The study provides a theoretical basis for grape planting and utilization of distinctive microorganisms in the wine industry.