摘要
目的探讨MPZL1在胃癌中的表达及其与胃癌患者预后的关系,以及MPZL1对胃癌细胞增殖、克隆形成能力的影响。方法利用GEPIA、UALCAN在线数据库分析MPZL1在多种恶性肿瘤中的表达情况,进一步运用KM Plotter和UALCAN数据库分析MPZL1对胃癌患者总生存时间的影响;采用Western blot检测胃癌细胞中MPZL1蛋白表达情况以及凋亡信号通路蛋白的变化;运用CCK-8和平板克隆实验分别检测MPZL1的表达变化对细胞增殖和克隆形成能力的影响。结果在线数据库GEPIA发现MPZL1在多种恶性肿瘤中呈高表达趋势。UALCAN和KM Plotter数据库分析结果显示MPZL1在胃癌组织中高表达,与胃癌患者的总生存时间呈负相关;CCK-8和平板克隆形成实验结果显示,过表达MPZL1促进胃癌细胞的增殖和克隆形成能力(P<0.05);Western blot结果显示,促凋亡蛋白Bad、Caspase-7表达水平在过表达组较对照组明显减少,在敲减组的表达水平高于对照组。结论MPZL1在胃癌组织中高表达,且促进胃癌细胞的增殖、克隆形成能力,可能与抑制凋亡信号通路有关。
Objective To investigate the expression of MPZL1 in gastric cancer tissues,its relation with the prognosis,and its effects on proliferation and colony formation of gastric cancer.Methods GEPIA and UALCAN databases were used to analyze the expression of MPZL1 in various malignant tumors.The KM Plotter database and UALCAN database were used to analyze the effect of MPZL1 on the overall survival of gastric cancer patients.The expression of MPZL1 protein and the changes of apoptosis-related proteins in gastric cancer cells were detected by Western blot,and the effects of MPZL1 expression on cell proliferation and colony formation were detected by CCK-8 and colony formation assay,respectively.Results MPZL1 was found to be highly expressed in various malignant tumors by GEPIA database.The results of UALCAN and KM Plotter databases analysis showed that MPZL1 was highly expressed in gastric cancer tissues,and might be correlated with the overall survival of gastric cancer patients.The results of CCK-8 and colony formation assay showed that the overexpression of MPZL1 promoted the proliferation and colony formation ability of gastric cancer cells(P<0.05).Western blot results showed that compared with control group,the expression levels of pro-apoptotic proteins Bad and Caspase-7 in the overexpression group were significantly lower while those in knockdown group were higher.Conclusion MPZL is highly expressed in gastric cancer tissues and promotes the proliferation and colony formation of gastric cancer cells,which may be related to the inhibition of apoptosis.
作者
林凤娟
葛潇潇
吴峥
唐文博
林莹
李进
LIN Fengjuan;GE Xiaoxiao;WU Zheng;TANG Wenbo;LIN Ying;LI Jin(Department of Oncology,Shanghai East Hospital,Tongji University,Shanghai 200000,China;Department of Radiotherapy Oncology,Renji Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China;Department of Medical Oncology,Fudan University Shanghai Cancer Center,Shanghai 200032,China)
出处
《肿瘤防治研究》
CAS
CSCD
2021年第7期679-685,共7页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金(81702360)
中央高校基本科研业务费专项资金(22120180596)。
关键词
MPZL1
胃癌
增殖
克隆
MPZL1
Gastric cancer
Proliferation
Colony
