PTEN和Eg5蛋白在前列腺癌中表达及相关性

Expression and correlation of PTEN and Eg5 protein in prostate cancer

目的对前列腺癌中PTEN和Eg5蛋白进行免疫组化检测,探讨PTEN和Eg5蛋白表达与前列腺癌临床病理的相关性。方法采用免疫组化SP法对2014年12月至2016年3月浙江大学医学院附属金华医院检测81例前列腺癌和45例前列腺增生PTEN和Eg5蛋白的表达,对前列腺癌临床病理资料与PTEN和Eg5表达分析,采用Spearmen对PTEN和Eg5蛋白表达进行相关性分析。结果前列腺癌中PTEN蛋白表达阳性率为25.93%(21/81),前列腺增生中表达阳性率为88.89%(40/45),二者比较,差异有统计学意义(P<0.05)。Eg5蛋白在前列腺癌中表达阳性率为70.37%(57/81),前列腺增生中表达阳性率为4.44%(2/45),二者比较,差异有统计学意义(P<0.05)。PTEN蛋白缺失表达和Eg5蛋白增高表达与前列腺癌的Gleason评分、TNM分期、术前PSA、精囊浸润、盆腔淋巴结转移和五年生化复发密切相关(P<0.05)。Spearmen相关性分析显示PTEN与Eg5蛋白的表达呈负相关(r=-0.763,P<0.05)。结论前列腺癌中PTEN蛋白下降表达,Eg5蛋白升高表达,它们异常表达与前列腺癌发生进展有关。

Objective To detect PTEN and Eg5 protein in prostate cancer(PC)by immunohistochemistry,and to explore the correlation between the expression of PTEN and Eg5 protein and clinicopathology of prostate cancer.Methods Immunohistochemical SP method was used to detect the expressions of PTEN and Eg5 protein in 81 PC cases and 45 cases with prostatic hyperplasia in Jinhua Hospital Affiliated to Zhejiang University School of Medicine from December 2014 to March 2016.The clinicopathological data of PC patients were analyzed with PTEN and Eg5 expression.Spearmen was used to analyze the correlation between PTEN and Eg5 protein expression.Results The positive rate of PTEN protein expression was 25.93%(21/81)in PC and 88.89%(40/45)in benign prostatic hyperplasia(BPH),with statistically significant difference(P<0.05).The positive rate of Eg5 protein was 70.37%(57/81)in PC and 4.44%(2/45)in BPH,with statistically significant difference(P<0.05).The loss of PTEN protein expression and the increased expression of Eg5 protein were closely related to Gleason score,TNM stage,preoperative PSA,seminal vesicle invasion,pelvic lymph node metastasis and five-year biochemical recurrence of PC(P<0.05).Spearmen correlation analysis showed that the expression of PTEN was negatively correlated with the expression of Eg5 protein(r=-0.763,P<0.05).Conclusion The expression of PTEN protein decreases and Eg5 protein increases in PC,and their abnormal expressions are related to the progression of PC.