基于网络药理学探讨华蟾素抗肿瘤的作用机制

Study on Mechanism of Cinobufotalin of Anti-Tumor Based on Network Pharmacological

目的:利用网络药理学分析方法,建立华蟾素抗肿瘤的"成分-靶点-通路"网络,并探究其潜在作用机制。方法:借助于CTD数据库寻找华蟾素抗肿瘤的潜在蛋白靶点,并通过Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库对获取的靶点进行相关通路分析,进一步利用Cytoscape3.7.0软件构建华蟾素的"成分-靶点-通路"网络关系图。结果:数据分析结果表明,华蟾素药物活性成分共30个,潜在作用蛋白靶点7个以及与肿瘤相关的KEGG通路15条。华蟾素抗肿瘤作用于BAX、BID、CASP9以及FAS等靶蛋白。其中,有6个蛋白靶点作用于p53信号通路、6个蛋白靶点作用于癌症通路,3个蛋白靶点作用于结直肠癌信号通路。结论:华蟾素在结直肠癌、非小细胞肺癌、前列腺癌等肿瘤以及抑癌通路、肿瘤坏死因子通路等方面均发挥重要作用。本研究为中医药在肿瘤靶向治疗、药理研究及临床应用可提供一定的理论基础。

Objective:To establish an anti-tumor component-target-pathway network of cinobufotalin and explore its potential mechanism by means of network pharmacological analysis.Methods:Potential anti-tumor protein targets of cinobufagin were identified by CTD database,and related pathways were analyzed by Kyoto Encyclopedia of Genes and Genomes(KEGG)database.Cytoscape 3.7.0 software was used to construct the"component-target-pathway"network diagram of cinobufagin.Results:Data analysis showed that there were 30 active ingredients,7 potential protein targets and 15 KEGG pathways related to cancer.Cinobufotalin acts on target proteins such as BAX,BID,CASP9 and FAS.Among them,6 protein targets act on p53 signaling pathway,6 protein targets act on cancer signaling pathway,and 3 protein targets act on colorectal cancer signaling pathway.Conclusions:Cinobufotalin plays an important role in colorectal cancer,nonsmall cell lung cancer,prostate cancer,tumor suppressor pathway and tumor necrosis factor pathway.This study provides a theoretical basis for the targeting therapy,pharmacological research and clinical application of traditional Chinese medicine in cancer.