摘要
利用大孔吸附树脂柱色谱、Sephdex LH-20凝胶柱色谱以及半制备高效液相色谱等技术,从连翘果实的乙醇提取物中分离得到三个新的化合物,包括两个苯乙醇苷类衍生物forsyside A(1),forsyside B(2)以及一个倍半萜forsysacid A(3).通过UV,IR,HRESIMS,一维NMR和二维NMR等波谱数据鉴定了它们的立体结构.化合物1和3的绝对构型通过比较实验和计算圆二色谱的方法进行了确定,化合物2的绝对构型通过激子手性法进行了确定.此外,对所有化合物的肝保护活性进行了研究,其中化合物2在10μmol·L^(-1)浓度下对N-乙酰基-对氨基苯酚(APAP)引起的肝细胞损伤表现出较强的抑制作用.
Three new compounds including two phenylethanoid glycosides derivatives,forsyside A(1)and forsyside B(2),together with a sesquiterpene forsysacid A(3)were isolated from the EtOH extract of the fruits of Forsythia suspensa.Their structures were elucidated from analysis of UV,IR,HRESIMS,and 1D/2D NMR data.The absolute configurations of 1 and 3 were determined by quantum electronic circular dichroism(ECD)calculation,and compound 2 was determined by the ECD exciton chirality method.In addition,all compounds were evaluated for their hepatoprotective activity against N-acetyl-paminophenol-induced(APAP)HepG2 cell injury,and compound 2 showed significant activity at a concentration of 10μmol·L^(-1).
作者
张旭
邵思远
冯子明
姜建双
杨桠楠
张培成
Zhang Xu;Shao Siyuan;Feng Ziming;Jiang Jianshuang;Yang Ya’nan;Zhang Peicheng(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100050)
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2021年第6期2496-2501,共6页
Chinese Journal of Organic Chemistry
基金
Project supported by the National Natural Science Foundation of China(No.81673313)
the Drug Innovation Major Project(No.2018ZX09711001-008)。
关键词
连翘
苯乙醇苷衍生物
倍半萜
肝保护活性
Forsythia suspensa
phenylethanoid glycosides derivative
sesquiterpene
hepatoprotective activity
