伸筋草经皮给药对小鼠抗炎镇痛作用及机制研究

Study on the Anti-inflammatory and Analgesic Effects and Mechanism of the Transdermal Administration of Shenjincao(Lycopodii Herba)on Mice

目的:探究伸筋草经皮给药后抗炎镇痛作用及其对小鼠血浆中TNF-ɑ、IL-1β、IL-6细胞因子水平的影响,探讨其作用机制。方法:伸筋草采用乙醇回流提取,测定提取物总黄酮含量,制备凝胶膏。小鼠随机分为模型组、青鹏软膏组和伸筋草高、低剂量组。小鼠脱毛后连续经皮给药10 d,采用蛋清致小鼠足跖肿胀、二甲苯致小鼠耳肿胀、甲醛致痛、醋酸扭体等模型测定伸筋草抗炎镇痛效果,应用ELISA法测定血浆中细胞因子TNF-ɑ、IL-1β、IL-6的水平。结果:与模型组比较,伸筋草低剂量组可明显抑制蛋清致炎后1、2、8 h小鼠足跖肿胀度(P<0.05或P<0.01),伸筋草高剂量组可明显抑制蛋清致炎后1 h小鼠足跖肿胀度(P<0.01);高、低剂量伸筋草均可明显抑制二甲苯致炎后小鼠耳肿胀度(P<0.01),且明显减少甲醛致痛后05 min小鼠舔足及缩足累积时间(P<0.05或P<0.01);伸筋草低剂量组小鼠扭体次数也明显减少(P<0.01);伸筋草高、低剂量组小鼠血浆IL-1β水平明显下降(P<0.05),伸筋草高剂量组小鼠血浆IL-6水平明显下降(P<0.05)。结论:伸筋草经皮给药具有较好的抗炎镇痛作用,其作用机制可能与抑制体内IL-1β与IL-6表达有关。

Objective:To explore the anti-inflammatory and analgesic effects of Shenjincao(Lycopodii herba after transdermal administration and its effects on the levels of TNF-ɑ,IL-1βand IL-6 cytokines in the plasma of mice,and to explore its mechanism of action.Method:Shenjincao(Lycopodii herba)was extracted with ethanol under reflux,and the total flavonoid content of the extract was determined to prepare a gel ointment.The mice were randomly divided into model group,Qingpeng ointment group,Shenjincao high dose group and Shenjincao low dose group.After depilation,mice were given continuous transdermal administration for 10 days.The antiinflammatory and analgesic effects of Shenjincao(Lycopodii herba)were tested by models such as egg white-induced swelling of mouse foot plantar,xylene-induced swelling of mouse ears,pain caused by formaldehyde,and writhing with acetic acid.The contents of TNF-α,IL-1βand IL-6 were examined by ELISA method.Results:Compared with the model group,the low dose of Shenjincao(Lycopodii herba)can significantly inhibit the swelling of the mouse foot plantar at 1,2,and 8 h after egg white-induced inflammation(P<0.05 or P<0.01).High dose Shenjincao(Lycopodii herba)can significantly inhibit the swelling degree of mouse foot plantar 1 h after egg white-induced inflammation(P<0.01).The ear swelling degree of mice after xylene-induced inflammation was significantly inhibited by high and low dose of Shenjincao(Lycopodii herba)(P<0.01),and the accumulated time of licking and shrinking feet in mice was significantly reduced 0-5 min after formaldehyde induced pain(P<0.05 or P<0.01).The writhing times of mice in Shenjincao low dose group were significantly reduced(P<0.01).Plasma IL-1βlevels of mice in Shenjincao high and low dose group were significantly decreased(P<0.05),and plasma IL-6 levels of mice in Shenjincao high dose group were significantly decreased(P<0.05).Conclusion:The transdermal administration of Shenjincao(Lycopodii herba)has a good anti-inflammatory and analgesic effect,and its mechanism may be related to inhibiting the expression of IL-1βand IL-6 in vivo.