SVF-gel局部注射对于皮肤光老化的改善研究

Improvement of local injection of SVF-Gel for skin photoaging

目的探讨基质血管成分(stromal vascular fraction,SVF)和基质血管成分胶(stromal vascular fraction gel,SVF-gel)局部皮下注射对光老化裸鼠皮肤的改善情况。方法选取建立成功的裸鼠皮肤光老化模型,选取脂肪组织20 ml,分别用于制备SVF和SVF-gel,以皮下注射的方式注入裸鼠背部光老化皮肤,观察治疗后1、2、4周裸鼠背部皮肤改善情况。结果应用SVF-gel、SVF治疗后,与对照组相比,治疗组背部皮肤的皱纹、质地、色素沉着、毛细血管扩张等方面均有显著的改善作用。H-E及MASSON染色显示:与对照组相比,治疗组表皮厚度增加,真皮层胶原纤维和弹性纤维排列整齐,数量增加。治疗后,裸鼠皮肤中丙二醛(malondialdehyde,MDA)含量降低,SVF-gel组MDA水平显著低于SVF组、对照组(P <0.05);谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)呈上升趋势,SVF-gel组GPX水平显著高于SVF组、对照组(P <0.05)。与对照组和SVF组相比,SVFgel治疗组胶原蛋白Ⅰ(collagen-Ⅰ,COL-Ⅰ)含量明显增加(P <0.05),治疗组细胞迁移诱导透明质酸结合蛋白(cell migration induced hyaluronan-binding protein,CEMIP)表达量下降(P <0.05)。结论 SVF、SVF-gel对小鼠皮肤光老化有明显的修复作用;SVF-gel改善作用优于SVF。

Objective To investigate the effects of stromal vascular fraction(SVF)and subcutaneous injection of SVF-gel on the skin of photoaging nude mice.Methods A nude mouse model of skin photoaging was successfully established.Then,20 ml adipose tissue was selected for the preparation of stromal vascular fraction(SVF)and SVF-gel,which were subcutaneously injected into the photoaging skin on the back of nude mice.The improvement of the back skin of the nude mice was observed one,two,and four weeks after treatment.Results After treatment with SVF-gel and SVF,the treatment group presented remarkable improvement in the wrinkles,texture,pigmentation,and telangiectasis of the back skin compared with the control group.According to HE and Masson staining,the treatment group showed increases in epidermal thickness and the number of collagen and elastic fibers in the dermis,compared with the control group.After treatment,the content of malondialdehyde(MDA)decreased in the skin of nude mice,while the MDA level in the SVF-gel group was significantly lower than those in the SVF group and the control group(P<0.05).The amounts of glutathione peroxidase(GPX)increased,while the level of GPX in the SVF-gel group was significantly higher than those in the SVF group and the control group(P<0.05).Compared with the control group and the SVF group,the content of collagen-I(Col-I)in the SVF-gel group significantly increased(P<0.05),while the expression of cell migration induced hyaluronan-binding protein(CEMIP)decreased in the treatment group(P<0.05).Conclusions SVF and SVF-gel have obvious repair effects on the mice with photoaging skin,where the improvement effect of SVF-gel is superior to SVF.