γ-氨基丁酸延缓失水胁迫下切花百合衰老的分析

Analysis onγ-Amino Butyric Acid Attenuates Senescence of Cut Lily under Dehydration Stress

为了研究γ-氨基丁酸(GABA)预处理对鲜切花百合在失水胁迫下的影响,本研究以温室采收的百合‘黄天霸’为材料,用适宜浓度的GABA预处理切花后进行失水胁迫。结果表明,与对照相比,失水胁迫导致切花百合出现褐化、花瓣脱落,MDA含量升高;而经GABA预处理,在失水胁迫下能延迟切花褐化和脱落的时间,延缓衰老。经生理生化检测,结果显示GABA不但能使MDA含量下降、TP含量升高,而且使超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)的活性升高,其中SOD反应最为敏感,而POD活性增加最大,APX则一直保持较高的活性。本研究结果表明了GABA预处理为延缓切花百合采后衰老、延长插瓶期提供了可能,并且暗示SOD、POD和APX在失水胁迫下延缓百合衰老中发挥了重要的作用。

In order to investigate the effects ofγ-amino butyric acid(GABA)pretreatment on the senescence of fresh cut lily,’Huang Tianba’were pretreated with a suitable solution GABA selected by preliminary experiments in this study,and then subjected to dehydration treatment.The results indicated that compared with the control,dehydration stress resulted in browning,shedding of the petals which led to premature senility and MDA content increasing;while pretreatment with GABA could effectively postpone browning,shedding and senescence under dehydration stress.The results of physiological and biochemical tests showed that GABA could not only reduce the content of MDA,raise the TP content,but increase the activities of SOD,CAT,POD,and APX.Among these enzymes,SOD was the most sensitive,POD activity increased sharply,APX maintained high activities.Our research demonstrated that GABA pretreatment provided the possibility to delay the post-harvest senescence of cut lilies and prolong the vase life.It seemed that SOD,POD and APX played important roles in attenuating senescence of cut lily under dehydration stress.