摘要
采用UPLC-PDA联合UPLC-MS/MS方法确证了未知药品中的卡那霉素。样品经提取稀释后,采用ACQUITY UPLC T3色谱柱为分离柱,UPLC-PDA初步筛查,再用正离子扫描,液相色谱串联质谱仪上机测定。结果显示,未知物确证是卡那霉素;样品检出限为0.1μg/mL,定量限为0.2μg/mL。本方法快速、灵敏、重现性好,适用于此批次未知药品中卡那霉素的检测。
UPLC-PDA combined UPLC-MS/MS method was used to confirm Kanamycin in unknown drugs.The sample was extracted and diluted,and the ACQUITY UPLC T3 chromatographic column was used as the separation column,UPLC-PDA preliminary screening,then positive ion scanning,liquid chromatography series mass spectrometer on the machine.The results showed that the unknown substance was Kanamycin,the detection limit was 0.1μg/mL,the quantitative limit was 0.2μg/mL.This method is rapid,sensitive and reproducible,and is suitable for the detection of Kanamycin in this batch of unknown drugs.
作者
魏秀丽
张传津
张琦
刘霄飞
张志民
杨志昆
WEI Xiu-li;ZHANG Chuan-jin;ZHANG Qi;LIU Xiao-fei;ZHANG Zhi-min;YANG Zhi-kun(Shandong Center for Quality Control of feed and Veterinary Drugs.Jinan 250100,China;Shandong Provincial Key Laboratory of Quality Safety Monitoring and Risk Assessment for Animal Products,Jinan 250100,China;China Shandong Engineering Laboratory for Drug Resistance Monitoring and Precise Drug Use of Animal Origin Bacteria,Jinan 250100,China;Shandong Provincial Center for Quality and Safety of Animal Products,jinan 250100,China)
出处
《中国兽药杂志》
2021年第7期41-47,共7页
Chinese Journal of Veterinary Drug
基金
2015年国家科技支撑计划“兽药安全与质量评价技术研究与应用”(BAD11B03-01)。
