帕金森病小鼠模型自主活动状态下的运动启动分析

Motion initiation analysis of voluntary movements in Parkinsonian mice

目的在自主活动状态下定义运动启动的指标,并分析帕金森病(Parkinson's disease,PD)小鼠模型的运动启动障碍。方法建立6-羟基多巴胺(6-hydroxydopamine,6-OHDA)损伤的PD小鼠模型,根据小鼠的运动速度划分出小鼠的静止、运动状态及相应的运动启动事件;将小鼠从静止到运动开始的间期定义为运动启动潜伏期(latency),并进行正常对照小鼠和PD小鼠的运动启动潜伏期比较。结果虽然PD小鼠模型的平均运动启动潜伏期与对照组小鼠比较差异无统计学意义,但其数据却呈偏态离散分布;继而按照运动启动潜伏期的不同时域特征进行分析,发现在潜伏期≤0.5 s范围内,PD模型小鼠与对照小鼠相比运动启动事件数量偏多,其潜伏期更短(P<0.05);在潜伏期>0.5 s时,PD小鼠较对照小鼠的潜伏期明显延长(P<0.05)。结论运动启动潜伏期可作为定义小鼠运动启动的有效指标,本实验为运动启动的研究提供了一种行为学参数。

Objective This research aimed to define a criterion for the motion initiation of voluntary movements and analyze the motion initiation dysfunction in mouse models of Parkinson’s disease(PD).Methods The Parkinsonian mouse models were established by 6-hydroxydopamine(6-OHDA)administration.The behavioral states were divided into the resting and the moving states,and the motion initiation events were identified according to the movement velocity of mice.The motion initiation latency was defined as the interim from the resting state to the moving state and was used to compare the difference between parkinsonian and control mice.Results Although there was no significant difference in the average motion initiation latency between parkinsonian mice and control mice,the data showed a skewed discrete distribution.Based on the distribution characteristics,we further analyzed the latency in different time domains.In the range of latency≤0.5 s,the number of motion initiation events was higher and the latency was shorter in parkinsonian mice,comparing with control mice(P<0.05).When latency>0.5 s,the latency of parkinsonian mice was significantly longer than that of control mice(P<0.05).Conclusion The motion initiation latency could be an effective criterion for evaluating the motion initiation of voluntary movements in mice.It provided a credible behavior paradigm for investigating the motion initiation dysfunction in PD.