摘要
A promising microalgal strain isolated from fresh water,which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting,was identified as Chlorella sp.USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid(rRNA)gene sequences.In the heterotrophic batch culture,more than 20.0 g·L^(-1)of cell dry weight concentration(DWC)of Chlorella sp.USTB-01 was obtained at day 5,and which was used directly to seed the autotrophic culture.A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp.USTB-01 for the fixation of carbon dioxide(CO_(2)).It showed that the autotrophic growth of Chlorella sp.USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L^(-1)was obtained at day 6.The highest CO_(2)fixation of 95%appeared on day 1 in the exponential growth phases of Chlorella sp.USTB-01 and 49.8%protein was found in the harvested microalgal cells.
基金
This research was supported by PetroChina Innovation Foundation(2009D-5006-04-02)
the Fundamental Research Funds for the Central Universities and the Metallurgical Foundation of University of Science and Technology Beijing.
