高糖条件下Exendin-4对骨髓间充质干细胞增殖分化的影响

The effects of Exendin-4 on the proliferation and differentiation of bone marrow mesenchymal stem cells under high glucose level

目的:观察艾塞那肽(Exendin-4)对高糖条件下骨髓间充质干细胞(BMSCs)增殖分化的影响。方法:分离培养SD大鼠BMSCs,高糖条件下用Exendin-4(1、5、10、15 nmol/L)干预,CCK-8检测细胞增殖;ALP染色、茜素红染色检测细胞成骨分化能力。结果:高糖可抑制BMSCs增殖(P<0.05),15 nmol/L Exendin-4可抑制BMSCs增殖(P<0.05),其他浓度则无明显影响;1 nmol/L Exendin-4对BMSCs ALP活性有增强作用(P<0.05);而15 nmol/L Exendin-4对BMSCs胞外矿化有抑制作用(P<0.05)。结论:高糖条件下Exendin-4在1~10 nmol/L浓度范围内对BMSCs的增殖无影响,但能促进其成骨分化,而高浓度(15 nmol/L)可抑制BMSCs的增殖和成骨分化。

Objective:To investigate the effects of Exendin-4 on the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs)in high glucose condition.Methods:The BMSCs of SD rats were cultured and passaged to P4-P6,the cells were cultured under low-glucose and high-glucose DMEM with Exendin-4 of 1,5,10 and 15 nmol/L respectively under the condition of high glucose.The BMSCs proliferation was assessed by CCK-8 kit on the 1st,3rd and 7th day of Exendin exposure,and the intracellular ALP activity was detected on the 7th day.The mineralization of BMSCs was tested with Alizaring red staining.Results:The proliferation of BMSCs was inhibited by high glucose(P<0.05).Exendin-4 of 15 nmol/L inhibited the proliferation of BMSCs on the 7th day(P<0.05).The ALP activity of BMSCs was enhanced by Exendin-4 of 1 nmol/L on the 14th day(P<0.05).Exendin-4 of 15 nmol/L inhibited the extracellular mineralization of BMSCs on the 14th day(P<0.05).Conclusion:Exendin-4 had no effect on the proliferation of BMSCs in the concentration range of 1-10 nmol/L under the condition of high glucose,while Exendin-4 of 15 nmol/L may inhibit both the proliferation and osteogenic differentiation of BMSCs.