基于生物信息学分析miR-655在口腔鳞状细胞癌中的表达及功能探讨

Analysis of the expression and function of miR-655 in oral squamous cell carcinoma based on bioinformatics

目的:基于生物信息学分析miR-655在口腔鳞状细胞癌中的表达及功能探讨。方法:下载TCGA数据库中的OSCC(n=342)及癌旁组织(n=44),同时收取湖北省恩施土家族苗族自治州中心医院口腔科手术切除的40例OSCC患者标本;比较miR-655的表达,分析miR-655的表达与OSCC预后的关系,体外实验初步探讨miR-655对OSCC细胞的影响。结果:miR-655在OSCC组低表达,miR-655的表达水平与肿瘤分级呈负相关,低表达组的总生存率更差,其表达水平是OSCC预后的独立因素(P<0.05)。体外实验表明miR-655抑制OSCC细胞的增殖、迁移及侵袭能力(P<0.05)。结论:miR-655在OSCC组织中低表达,可作为OSCC预后评估的潜在标记物。

Objective:To investigate the expression and function of miR-655 in oral squamous cell carcinoma(OSCC)based on bioinformatic.Methods:The OSCC(n=342)and OSCC adjacent tissue(OAT,n=44)were searched cohort data from TCGA database,and the OSCC tissue samples of 40 patients were obtained in the central hospital of Enshi autonomous prefecture.The expression of miR-655 were estimated between OSCC and control groups.Then the association of miR-655 expression with OSCC prognosis was analyzed.Finally,the effects of miR-655 on OSCC were explored by in vitro experiments.Results:The expression of miR-655 was lower in OSCC than in OAT(P<0.001)and was negatively related with tumor grade(P=0.044),The samples with low miR-655 expression showed a poor prognosis(Log-rank P=1.19E-02).Cox regression analysis showed that the expression of miR-655 in OSCC was an independent predictor for prognosis(P=0.020).Functional assays demonstrated that miR-655 inhibited OSCC cell proliferation,migration and invasion(P<0.05).Conclusion:miR-655 expression is low in OSCC,and may be used as a potential prognostic biomarker for OSCC.