丹酚酸A治疗在体腰椎小关节骨性关节炎的实验研究

Efficacy of Salvianolic Acid A on Lumbar FacetJoint Osteoarthritis in Rabbits in Vivo

目的:观察丹酚酸A对持续压力导致的腰椎小关节骨性关节炎的干预作用。方法:采用在体加压装置,建立在体兔脊柱运动节段压力退变模型。将24只新西兰白兔随机分为对照组、模型组与丹酚酸组,其中模型组在L4~5节段予以持续294 kPa压力,丹酚酸组在持续压力基础上予腹腔注射丹酚酸A干预。术后第28天各组切取双侧L4~5小关节,分离关节软骨进行番红固绿染色,采用流式细胞术测定软骨细胞凋亡情况,分离滑膜组织采用qPCR与Western blot法检测滑膜组织炎症因子表达情况。结果:模型组腰椎小关节软骨表面形态不平整,出现裂隙,丹酚酸组软骨表面相对光滑,但染色欠均匀。模型组软骨细胞显著凋亡,而丹酚酸组则下调了该模型中的凋亡比例,差异有统计学意义(P<0.05)。相比模型组,丹酚酸干预后的腰椎小关节滑膜组织中炎症因子IL-1β、TNF-α、IL-6的mRNA与蛋白表达水平出现显著降低,差异有统计学意义(P<0.05)。结论:丹酚酸A能够缓解持续压力对腰椎小关节软骨造成的损伤程度,抑制软骨细胞凋亡与滑膜组织中炎症因子的表达。

Objective:To observe the efficacy of salvianolic acid A on lumbar facet joint osteoarthritis caused by constant compression.Methods: In-vivo compression device was used to establish the constant compression model of rabbit spinal motion segment.24 New Zealand white rabbits were randomly divided into controls, the load group, and the salvianolic acid group.The load group was given constant compression of 294 kPa at the lumbar 4/5 segment, and the salvianolic acid group was given an intraperitoneal injection of salvianolic acid A based on constant compression.On the 28 th day after the operation, bilateral L4/L5 facets were harvested from each group.The articular cartilage was separated and stained with safranin and solid green.The apoptosis of chondrocytes was detected by flow cytometry.The expression of inflammatory factors of IL-1β、TNF-α、IL-6 in synovial tissue was detected by qPCR and Western Blot.Results: The surface of the articular cartilage of lumbar facet joints was uneven with cracks in the load group.The surface of cartilage was relatively smooth in the salvianolic acid group, but the staining was not uniform.Chondrocytes in the load group were significantly apoptotic, while the salvianolic acid group decreased the proportion of apoptosis in the model(P<0.05).Compared with the model group, the mRNA and Western Blot expression levels of inflammatory factors IL-1 β, TNF-α, and IL-6 in lumbar facet joint synovial tissue were significantly decreased(P<0.05) after the salvianolic acid intervention.Conclusion: Salvianolic acid A can alleviate the damage of lumbar facet articular cartilage caused by constant compression, inhibit chondrocyte apoptosis, and the expression of inflammatory factors in synovial tissue.