瑞香素对去卵巢大鼠炎性因子和骨代谢的影响

Efficacy of Daphnetin on Inflammatory Factors andBone Metabolism in Ovariectomized Rats

目的:探讨瑞香素对去卵巢大鼠炎性因子和骨代谢指标的影响。方法:将60只3个月龄SD雌性大鼠随机分为低、中、高剂量组,对照组、模型组、空白组,每组10只。低、中、高剂量组分别予以瑞香素0.275、 0.463、0.668 mg/kg灌胃,1次/d;对照组阿仑膦酸钠剂量0.630 mg/kg, 1次/周;模型组0.9%生理盐水0.668 mg/kg灌胃;空白组不干预;连续给药12周,12周后生化法检测血清钙(Ca)、磷(P)、碱性磷酸酶(ALP),ELISA法检测白介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)的水平;治疗前后运用双能X线法测全身骨密度(BMD)。结果:治疗前空白组大鼠BMD高于其他各组,差异有统计学意义(P<0.05);治疗后高剂量组和对照组大鼠的BMD均高于模型组,差异有统计学意义(P<0.05)。低剂量组、高剂量组和空白组IL-6含量均低于模型组,差异有统计学意义(P<0.05);低剂量组、高剂量组和对照组和空白组TNF-α的含量均低于模型组,差异有统计学意义(P<0.05)。高剂量组Ca含量高于对照组和模型组。低剂量组、高剂量组P含量均高于模型组,差异有统计学意义(P<0.05)。中剂量组、高剂量组、对照组ALP含量均低于模型组,差异有统计学意义(P<0.05)。结论:瑞香素高剂量能够提高绝经后骨质疏松大鼠的BMD,降低IL-6和TNF-α的水平,发挥抗炎作用;提高绝经后骨质疏松大鼠Ca和P的含量,降低ALP的含量,改善骨代谢。瑞香素可改善去卵巢骨质疏松大鼠的骨痛,增加骨密度和改善骨代谢,对骨质疏松症起治疗作用。

Objective:To investigate the efficacies of daphnetin on inflammatory factors and bone metabolism in ovariectomized rats.Methods: 60 three-month-old SD rats were randomly divided into low, medium and high dose groups, control group, model group and blank group, with 10 rats in each group.Low, medium and high dose groups were given daphnetin 0.275, 0.463 and 0.668 mg/kg by gavage, respectively.Once a day.The dose of Allen phosphonic acid sodium in control group was 0.630 mg/kg.Once a week.The model group was given 0.9% saline 0.668 mg/kg by gavage.The blank group was not intervened.All groups were administered continuously for 12 weeks.After 12 weeks, serum calcium(Ca), phosphorus(P), alkaline phosphatase(ALP) was detected by the biochemical method to detect.Interleukin 6(IL-6), tumor necrosis factor-α(TNF-α) level were detected by ELISA method.Total bone mass density(BMD) was measured by double energy X method before and after treatment.Results: Before treatment, BMD of rats in the blank group was higher than that of other groups(P<0.05).After treatment, BMD of rats in each group was higher than that of the model group(P<0.05).After administration, the BMD of rats in the high-dose group and the control group was higher than before administration.The IL-6 content in the low dose, high dose and blank group was lower than that in the model group(P<0.05).The levels of TNF-α in the low dose, high dose, control group and blank group were all lower than those in the model group(P<0.05).The Ca content in the high-dose group was higher than that in the control group and the model group.The P content in the low dose group and the high dose group was higher than that in the model group(P<0.05).The ALP content of medium dose group, high dose group and control group was lower than that of model group(P<0.05).Conclusion: High doses of daphnetin can increase BMD in postmenopausal osteoporosis rats, reduce the level of IL-6 and TNF-α to play a role of anti-inflammatory, enhance the content of Ca and P in postmenopausal osteoporosis rats, reduce the content of ALP to improve bone metabolism.Daphnetin can improve bone pain, increase bone density and improve bone metabolism in ovariectomized osteoporosis rats, and play a therapeutic role in osteoporosis.