CLEC5A抑制肝胆管细胞癌增殖和转移的作用研究

The inhibitory effect of CLEC5A on cell proliferation and metastasis in intrahepatic cholangiocarcinoma

目的探究C型凝集素结构域家族5成员A(CLEC5A)在肝胆管细胞癌(ICC)组织中的表达特点,并分析其对ICC细胞株RBE增殖、凋亡、迁移以及侵袭特性的影响。方法免疫组化染色法检测25例ICC患者癌组织及配对癌旁组织中CLEC5A的表达水平;构建慢病毒介导CLEC5A过表达RBE细胞株,采用实时荧光定量PCR检测细胞内CLEC5A表达水平,CCK-8法检测CLEC5A对RBE细胞增殖能力的影响,流式细胞仪检测RBE细胞凋亡和细胞周期,Transwell法检测细胞迁移及侵袭能力。结果CLEC5A在ICC癌组织中的表达水平明显低于癌旁组织(P<0.05)。过表达CLEC5A可以抑制RBE细胞的增殖、迁移及侵袭能力,诱导细胞凋亡并引起G2/M期阻滞(P<0.05)。结论CLEC5A是一个潜在的ICC抑癌基因,它可能通过调控细胞增殖、迁移及侵袭特性影响ICC发生发展。

Objective To detect the expression of CLEC5A in intrahepatic cholangiocarcinoma(ICC)tissues and analyze its effect on cell proliferation,apoptosis,migration and invasion of RBE cells.Methods The expression levels of CLEC5A were assessed by immunohistochemistry(IHC)in 25 samples of ICC and adjacent normal tissues.RBE cells were transfected with lentiviral vector overexpressing CLEC5A,and qPCR was used to detect the expression levels of CLEC5A.CCK-8 was used to detect the proliferation,flow cytometric analysis was used to detect cell apoptotic proportion and cell cycle distribution,and Transwell assay was performed to detect cell migration and invasion in RBE cells.Results IHC score was significantly lower in tumor tissues than that in the adjacent normal tissues(P<0.05).Overexpression of CLEC5A inhibited cell proliferation,migration and invasion and induced cell apoptosis and led to G2/M phase arrestment in RBE cells(P<0.05).Conclusion CLEC5A is a potential ICC cancer suppressor gene,which may affect the development of ICC by regulating cell proliferation,metastasis and invasion.