浙江近年育成粳稻新品种(系)部分抗病虫基因的分子检测与育种应用

Molecular detection and breeding application of some disease and insect resistance genes of japonica rice varieties/lines recently developed in Zhejiang Province

为明确浙江省近年育成常规晚粳稻品种(系)的稻瘟病、白叶枯病、褐飞虱等抗性基因的分布情况,利用分子标记检测分析了11个抗稻瘟病基因(Pita、Pib、Pigm、Pikh/Pi54、Pi9、Pi1、Pi2、Pi5、Pit、Pb1、Pikm),2个抗白叶枯基因(Xa21、Xa23)和2个抗褐飞虱基因(Bph14、Bph15)在61个晚粳稻品种(系)分布情况。结果表明:稻瘟病抗性基因检出率最高的为Pib,分布频率为91.80%,Pikm、Pi2、Pita的分布频率分别为68.85%、67.21%、63.93%,Pit、Pi5、Pi9、Pikh/Pi54检出频率分别为34.43%、32.79%、26.23%、24.59%;Pb1、pigm检出率较低,分别为6.56%、1.64%;Pi1基因未检出;抗褐飞虱基因Bph14、Bph15检出率分别为6.56%、11.48%;抗白叶枯基因Xa21、Xa23在61个被检测品种(系)中无分布。利用分子标记辅助选择将Xa23、Bph14、Bph15导入粳稻中并验证了抗性,结果表明,以上基因均具有较好的抗性。初步明确了浙江省新育成61个常规晚粳稻品种(系)的抗病虫基因分布情况,研究结果将为今后利用分子标记辅助技术将目标抗性基因转移到待改良品种中培育广谱多抗水稻新品种提供依据。

In order to clarify the distribution of some disease and insect resistance genes of japonica rice varieties/lines recently developed in Zhejiang Province,in this study,the distribution of blast resistance gene Pita,Pib,Pigm,Pi9,Pi1,Pikh/Pi54,Pi2,Pi5,Pit,Pb1,Pikm and BPH resistance gene Bph14,Bph15,bacterial blight resistance gene Xa21,Xa23 in 61 japonica rice varieties/lines were analyzed.The results showed that the highest detection frequency of rice blast resistance gene was Pib,the distribution frequency was 91.80%,the distribution frequency of Pikm,Pi2 and Pita were 68.85%,67.21%,63.93%,respectively;the distribution frequency of Pit,Pi5,Pi9,Pikh/Pi54 were 34.43%,32.79%,26.23%and 24.59%,respectively.The distribution frequency of Pb1 and Pigm were 6.56%,1.64%,respectively.The Pi1 was not distributed in 61 tested varieties/lines.The distribution frequency of Bph14 and Bph15 were 6.56%and 11.48%,respectively.The bacterial blight resistance gene Xa21 and Xa23were not distributed in 61 tested varieties/lines.In this study,Xa23,Bph14 and Bph15 were introduced into japonica rice by marker assisted selection,and the results showed that the above genes had good resistance.In this study,the distribution of resistance genes of 61 japonica rice varieties/lines in Zhejiang Province were preliminarily established,the results of this study will provide a scientific basis for transferring the target resistance genes into the varieties to be improved and breeding new varieties with broad-spectrum and multi resistance.