Th1/Th2平衡对寒凝血瘀证原发性痛经大鼠血清PG、COX-2、AVP及OT的影响

Effect of Th1/Th2 balance on serum prostaglandin, COX-2, AVP and OT in primary dysmenorrhea rats with cold coagulation and blood stasis syndrome

目的:通过研究Th1/Th2平衡漂移对寒凝血瘀证原发性痛经(primary dysmenorrhea,PD)大鼠血清前列腺素F_(2α)(prostaglandin F_(2α),PGF_(2α))、前列腺素E_(2)(prostaglandin E_(2),PGE_(2))、6-酮-前列腺素F_(1α)(6-Keto-Prostaglandin F_(1α),6-Keto-PGF_(1α))、血栓素B2(thromboxane B2,TXB2)、环氧合酶-2(cyclooxygenase-2,COX-2)、血管加压素(arginine vasopressin,AVP)及缩宫素(oxytocin,OT)的影响,探讨Th1/Th2平衡在PD发病中的作用。方法:建立寒凝血瘀证PD大鼠模型,在此模型基础上建立Th1偏移PD模型及Th2偏移PD模型,即分为:空白组、模型组、Th1偏移组、Th2偏移组。比较各组大鼠扭体反应,检测各组大鼠血清PGF_(2α)、PGE_(2)、6-Keto-PGF_(1α)、TXB2、COX-2、AVP及OT含量。结果:Th1、Th2偏移组扭体潜伏时间与模型组比较,差异均无统计学意义(P>0.05);Th2偏移组扭体潜伏时间较Th1偏移组缩短,差异具有统计学意义(P<0.05)。与模型组比较,Th1偏移组30 min内扭体次数明显减少,而Th2偏移组30 min内扭体次数明显增加,差异均具有统计学意义(P<0.01)。与空白组比较,各组PGF_(2α)、TXB2、COX-2、AVP及OT含量均升高,6-Keto-PGF_(1α)及PGE_(2)含量降低,差异均有统计学意义(P<0.05)。与模型组比较,Th1偏移组PGF_(2α)、TXB2、COX-2及AVP含量均降低,6-Keto-PGF_(1α)、PGE_(2)含量升高,差异均有统计学意义(P<0.05);与模型组比较,Th2偏移组PGF_(2α)、TXB2、COX-2、AVP及OT含量均升高(P<0.01),6-Keto-PGF_(1α)、PGE_(2)含量降低,差异均有统计学意义(P<0.01)。结论:Th1/Th2细胞平衡干预PGF_(2α)、PGE_(2)、6-Keto-PGF_(1α)、TXB2、COX-2、AVP及OT表达可能是寒凝血瘀证PD的发病机制之一。

Objective:To explore the role of Th1/Th2 balance in the pathogenesis of primary dysmenorrhea through study the effect of Th1/Th2 balance drift on serum prostaglandin F_(2α),prostaglandin E_(2),6-keto-prostaglandin F_(1α),TXB2,COX-2,AVP and OT in primary dysmenorrhea rats with cold coagulation and blood stasis syndrome. Method:The rat model of primary dysmenorrhea with cold coagulation and blood stasis syndrome was established. On the basis of this model,Th1 shift model and Th2 shift model were established. The rats were divided into blank group,model group,Th1 shifting group and Th2 shifting group. The writhing reaction of rats in each group was compared,and the contents of PGF_(2α),PGE_(2),6-keto-PGF_(1α),TXB2,COX-2,AVP and OT in serum were detected. Results:Writhing latency:There was no significant difference between Th1 and Th2 shifting groups(P>0.05),Th2 shifting group was shorter than Th1 shifting group(P<0.05). Writhing times in 30 minutes:Compared with the model group,the writhing times of Th1 shifting group decreased significantly(P<0.01),while Th2 shifting group increased significantly(P<0.01).Compared with the blank group,the contents of PGF_(2α),TXB2,COX-2,AVP and OT were increased(P<0.05,P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2) were decreased(P<0.01). Compared with the model group,the contents of PGF_(2α),TXB2,COX-2 and AVP in Th1 shifting group were decreased(P<0.05,P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2) were increased(P<0.01),while the contents of PGF_(2α),TXB2,COX-2,AVP and OT in Th2 shifting group were increased(P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2) were decreased(P<0.01).Conclusion:The intervention of Th1/Th2 cell balance on the expression of PGF_(2α),PGE_(2),6-keto-PGF_(1α),TXB2,COX-2,AVP and OT may be one of the pathogenesis of PD with cold coagulation and blood stasis syndrome.