电针针刺内关、郄门穴对急性心肌缺血模型大鼠心肌能量代谢的影响

Effects of electroacupuncture of acupoints of the neiguan and ximen on ischemic myocardium energy metabolism with acute myocardial ischemia rats

目的:观察电针针刺内关、郄门穴对冠脉结扎所致的急性心肌缺血大鼠模型的心肌能量代谢的影响。方法:将40只标准SD大鼠随机分为内关组、郄门组、模型组、假手术组。采用冠脉结扎的方法制备急性心肌缺血大鼠模型,假手术组只穿线不结扎。造模成功后,内关组电针针刺内关穴、郄门组电针针刺郄门穴。模型组与假手术组不予以电针针刺治疗,于每天同一时间使用与内关组及郄门组相同的方法进行抓取和固定,每天1次,连续14 d。实验结束后,采用HE染色法观察各组大鼠心肌病理学的动态变化、试剂盒检测游离脂肪酸(FFA)、三磷酸腺苷(ATP)含量和Na^(+)-K^(+)-ATP酶活力。结果:病理结果显示,针刺郄门、内关可改善缺血心肌细胞损伤;与假手术组相比,模型组FFA增高,ATP含量和Na^(+)-K^(+)-ATP酶活力降低,差异均具有统计学意义(P<0.01);与模型组相比,郄门组、内关组FFA减少,ATP含量和Na^(+)-K^(+)-ATP酶活力增高,差异均具有统计学意义(P<0.05或P<0.01)。结论:电针针刺手厥阴心包经腧穴可以改善缺血心肌细胞损伤,改善心肌细胞能量代谢。

Objective:To observe the effect of Electroacupuncture of Acupoints of the Neiguan(PC6)and Ximen(PC4)on its myocardial energy metabolism with acute myocardial ischemia rats caused by coronary artery ligation. Method:Forty standard Spargue-Ddwely(SD)rats were divided into forty groups randomly,namely,group of Neiguan,group of Ximen,model group,sham operation group. The group of rats of myocardial ischemia were induced by coronary ligation,and the model rat of acute myocardial ischemia was prepared. The sham operation grouphad no ligation of the needle. After anesthesia was awakened,acupuncture was applied bilateral at Neiguan and Ximen of the group of Neiguan and Ximen,once a day at a fixed time for 14 days.The rats of model group and the sham operation group were not given acupunctured,but use the samely methos of Neiguan and Ximen groups to grap the rats at the same time everyday.After 14 days the experiment was over,observed the pathological changes of myocardial tissue by HE staining,detected the levels of free fatty acid(FFA),adenosine triphosphate(ATP)content,and the activities of Na^(+)-K^(+)-ATP enzyme according to the reagents’ demand. Result:Pathological results showed that acupuncuted at Neiguan and Ximen could improve the ischemic cardiomyocyte injury. Compared with the sham operation group,the levels of FFA in model group were increased(P<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATP enzyme were all decreased(P<0.01);Compared with the model group,the levels of FFA were decreased(P<0.05,P<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATP enzyme were improved. Conclusion:Acupuncturing at the Pericardium Meridian can alleviate the cell injury by myocardial ischemia,and also can improve the myocardial energetic metabolism in acute myocardial ischemia.